Molecular Cloning and Characterization of the Calcineurin Subunit A from Plutella xylostella
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cloning and Sequence Analysis of PxCNA
2.2. Developmental Stage and Tissue-Specific Expression Patterns
2.3. Heterologous Expression and Purification of Recombinant Proteins
2.4. Properties of rPxCNA and rPxCNα
2.5. Effect of Pyrethroids and Phosphatase Inhibitors on rPxCNα
3. Experimental Section
3.1. Insects and Chemicals
3.2. Cloning and Sequence Analysis of PxCNA
3.3. Stage- and Tissue-Specific Expression of PxCNA
3.4. E. coli Expression and Purification
3.5. Phosphatase Activity Determination In Vitro
3.6. In Vitro Inhibition Assay
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Chen, X.; Zhang, Y. Molecular Cloning and Characterization of the Calcineurin Subunit A from Plutella xylostella. Int. J. Mol. Sci. 2013, 14, 20692-20703. https://doi.org/10.3390/ijms141020692
Chen X, Zhang Y. Molecular Cloning and Characterization of the Calcineurin Subunit A from Plutella xylostella. International Journal of Molecular Sciences. 2013; 14(10):20692-20703. https://doi.org/10.3390/ijms141020692
Chicago/Turabian StyleChen, Xi'en, and Yalin Zhang. 2013. "Molecular Cloning and Characterization of the Calcineurin Subunit A from Plutella xylostella" International Journal of Molecular Sciences 14, no. 10: 20692-20703. https://doi.org/10.3390/ijms141020692