Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2013, 14(10), 20635-20657; doi:10.3390/ijms141020635
Review

Mass Spectrometry Coupled Experiments and Protein Structure Modeling Methods

1,2
 and 1,2,*
Received: 30 July 2013; in revised form: 17 September 2013 / Accepted: 19 September 2013 / Published: 15 October 2013
(This article belongs to the Special Issue Protein Folding 2015)
View Full-Text   |   Download PDF [545 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: With the accumulation of next generation sequencing data, there is increasing interest in the study of intra-species difference in molecular biology, especially in relation to disease analysis. Furthermore, the dynamics of the protein is being identified as a critical factor in its function. Although accuracy of protein structure prediction methods is high, provided there are structural templates, most methods are still insensitive to amino-acid differences at critical points that may change the overall structure. Also, predicted structures are inherently static and do not provide information about structural change over time. It is challenging to address the sensitivity and the dynamics by computational structure predictions alone. However, with the fast development of diverse mass spectrometry coupled experiments, low-resolution but fast and sensitive structural information can be obtained. This information can then be integrated into the structure prediction process to further improve the sensitivity and address the dynamics of the protein structures. For this purpose, this article focuses on reviewing two aspects: the types of mass spectrometry coupled experiments and structural data that are obtainable through those experiments; and the structure prediction methods that can utilize these data as constraints. Also, short review of current efforts in integrating experimental data in the structural modeling is provided.
Keywords: constraint-base structure prediction; integrative structure prediction; sequence variants; protein dynamics; mass spectrometry constraint-base structure prediction; integrative structure prediction; sequence variants; protein dynamics; mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Pi, J.; Sael, L. Mass Spectrometry Coupled Experiments and Protein Structure Modeling Methods. Int. J. Mol. Sci. 2013, 14, 20635-20657.

AMA Style

Pi J, Sael L. Mass Spectrometry Coupled Experiments and Protein Structure Modeling Methods. International Journal of Molecular Sciences. 2013; 14(10):20635-20657.

Chicago/Turabian Style

Pi, Jaewoo; Sael, Lee. 2013. "Mass Spectrometry Coupled Experiments and Protein Structure Modeling Methods." Int. J. Mol. Sci. 14, no. 10: 20635-20657.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert