Int. J. Mol. Sci. 2013, 14(10), 20555-20563; doi:10.3390/ijms141020555
Article

The Key Enzyme of the Sialic Acid Metabolism Is Involved in Embryoid Body Formation and Expression of Marker Genes of Germ Layer Formation

Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Hollystr.1, Halle D-06114, Germany
* Author to whom correspondence should be addressed.
Received: 28 August 2013; in revised form: 24 September 2013 / Accepted: 27 September 2013 / Published: 14 October 2013
(This article belongs to the Special Issue Glycosylation and Glycoproteins)
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Abstract: The bi-functional enzyme UDP-N-acetyl-2-epimerase/N-acetylmannosamine kinase (GNE) is the key enzyme of the sialic acid biosynthesis. Sialic acids are negatively charged nine carbon amino sugars and are found on most glycoproteins and many glycolipids in terminal positions, where they are involved in a variety of biological important molecular interactions. Inactivation of the GNE by homologous recombination results in early embryonic lethality in mice. Here, we report that GNE-deficient embryonic stem cells express less differentiation markers compared to wild-type embryonic stem cells. As a result, GNE-deficient embryonic stem cells fail to form proper embryoid bodies (EB) within the first day of culture. However, when culturing these cells in the presence of sialic acids for three days, also GNE-deficient embryonic stem cells form normal EBs. In contrast, when culturing these cells in sialic acid reduced medium, GNE-deficient embryonic stem cells proliferate faster and form larger EBs without any change in the expression of markers of the germ layers.
Keywords: sialic acid biosynthesis; UDP-N-acetyl-2-epimerase/N-acetylmannosamine kinase; embryonic stem cells; embryoid bodies; gene expression

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MDPI and ACS Style

Weidemann, W.; Hering, J.; Bennmann, D.; Thate, A.; Horstkorte, R. The Key Enzyme of the Sialic Acid Metabolism Is Involved in Embryoid Body Formation and Expression of Marker Genes of Germ Layer Formation. Int. J. Mol. Sci. 2013, 14, 20555-20563.

AMA Style

Weidemann W, Hering J, Bennmann D, Thate A, Horstkorte R. The Key Enzyme of the Sialic Acid Metabolism Is Involved in Embryoid Body Formation and Expression of Marker Genes of Germ Layer Formation. International Journal of Molecular Sciences. 2013; 14(10):20555-20563.

Chicago/Turabian Style

Weidemann, Wenke; Hering, Jessica; Bennmann, Dorit; Thate, Annett; Horstkorte, Rüdiger. 2013. "The Key Enzyme of the Sialic Acid Metabolism Is Involved in Embryoid Body Formation and Expression of Marker Genes of Germ Layer Formation." Int. J. Mol. Sci. 14, no. 10: 20555-20563.

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