Next Article in Journal
Targeting of Rho Kinase Ameliorates Impairment of Diabetic Endothelial Function in Intrarenal Artery
Next Article in Special Issue
RNA-Binding Proteins Impacting on Internal Initiation of Translation
Previous Article in Journal
Acidosis Decreases c-Myc Oncogene Expression in Human Lymphoma Cells: A Role for the Proton-Sensing G Protein-Coupled Receptor TDAG8
Previous Article in Special Issue
Disease Animal Models of TDP-43 Proteinopathy and Their Pre-Clinical Applications
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(10), 20256-20281; doi:10.3390/ijms141020256

HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes

Division of Biological Research and Biotechnology, Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, 48 Vas. Constantinou Avenue, Athens 11635, Greece
Laboratory of Biotechnology, School of Chemical Engineering, National Technical University of Athens, Athens 15780, Greece
Authors to whom correspondence should be addressed.
Received: 29 July 2013 / Revised: 6 September 2013 / Accepted: 16 September 2013 / Published: 11 October 2013
View Full-Text   |   Download PDF [562 KB, uploaded 19 June 2014]   |  


Post-transcriptional regulatory networks are dependent on the interplay of many RNA-binding proteins having a major role in mRNA processing events in mammals. We have been interested in the concerted action of the two RNA-binding proteins hnRNP A1 and HuR, both stable components of immunoselected hnRNP complexes and having a major nuclear localization. Specifically, we present here the application of the RNA-immunoprecipitation (RIP)-Chip technology to identify a population of nuclear transcripts associated with hnRNP A1-RNPs as isolated from the nuclear extract of either HuR WT or HuR-depleted (KO) mouse embryonic fibroblast (MEF) cells. The outcome of this analysis was a list of target genes regulated via HuR for their association (either increased or reduced) with the nuclear hnRNP A1-RNP complexes. Real time PCR analysis was applied to validate a selected number of nuclear mRNA transcripts, as well as to identify pre-spliced transcripts (in addition to their mature mRNA counterpart) within the isolated nuclear hnRNP A1-RNPs. The differentially enriched mRNAs were found to belong to GO categories relevant to biological processes anticipated for hnRNP A1 and HuR (such as transport, transcription, translation, apoptosis and cell cycle) indicating their concerted function in mRNA metabolism.
Keywords: RNA-binding proteins (RBPs); ribonucleoprotein (hnRNP) complexes; post-transcriptional regulation; mRNA processing; RNA-immunoprecipitation (RIP)-Chip technology; mouse embryonic fibroblasts (MEFs) RNA-binding proteins (RBPs); ribonucleoprotein (hnRNP) complexes; post-transcriptional regulation; mRNA processing; RNA-immunoprecipitation (RIP)-Chip technology; mouse embryonic fibroblasts (MEFs)

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary materials

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Papadodima, O.; Chatziioannou, A.; Patrinou-Georgoula, M.; Kolisis, F.N.; Pletsa, V.; Guialis, A. HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes. Int. J. Mol. Sci. 2013, 14, 20256-20281.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top