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Int. J. Mol. Sci. 2013, 14(10), 19911-19922; doi:10.3390/ijms141019911
Article

Inhibiting Invasion into Human Bladder Carcinoma 5637 Cells with Diallyl Trisulfide by Inhibiting Matrix Metalloproteinase Activities and Tightening Tight Junctions

1
, 2
, 3
, 4
 and 5,6,*
1 Dongnam Institute of Radiological & Medicine Sciences, Busan 619-953, Korea 2 Departments of Parasitology and Genetics, College of Medicine, Kosin University, Busan 602-702, Korea 3 Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea 4 Department of Urology, College of Medicine, Chungbuk National University, Cheongju 361-763, Korea 5 Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University, Busan 614-714, Korea 6 Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 614-052, Korea
* Author to whom correspondence should be addressed.
Received: 15 July 2013 / Revised: 9 September 2013 / Accepted: 23 September 2013 / Published: 1 October 2013
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

Diallyl trisulfide (DATS), an organosulfur compound in garlic, possesses pronounced anti-cancer potential. However, the anti-invasive mechanism of this compound in human bladder carcinoma is not fully understood. In this study, we evaluated the anti-invasive effects of DATS on a human bladder carcinoma (5637) cell line and investigated the underlying mechanism. The results indicated that DATS suppressed migration and invasion of 5637 cells by reducing the activities and expression of matrix metalloproteinase (MMP)-2 and MMP-9 at both the protein and mRNA levels. DATS treatment up-regulated expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in 5637 cells. The inhibitory effects of DATS on invasiveness were associated with an increase in transepithelial electrical resistance and repression of the levels of claudin family members. Although further studies are needed, our data demonstrate that DATS exhibits anti-invasive effects in 5637 cells by down-regulating the activity of tight junctions and MMPs. DATS may have future utility in clinical applications for treating bladder cancer.
Keywords: DATS; invasion; MMP; tight junction DATS; invasion; MMP; tight junction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Shin, D.Y.; Cha, H.-J.; Kim, G.-Y.; Kim, W.-J.; Choi, Y.H. Inhibiting Invasion into Human Bladder Carcinoma 5637 Cells with Diallyl Trisulfide by Inhibiting Matrix Metalloproteinase Activities and Tightening Tight Junctions. Int. J. Mol. Sci. 2013, 14, 19911-19922.

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