Int. J. Mol. Sci. 2012, 13(9), 11832-11843; doi:10.3390/ijms130911832
Article

Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor

Fujun Yao 1 email, Ruiping Zhang 2,* email, He Tian 2 email and Xiangjun Li 1,* email
1 College of Chemistry and Chemical Engineering, Graduate University, Chinese Academy of Sciences, Beijing, 100049, China 2 Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
* Authors to whom correspondence should be addressed.
Received: 18 July 2012; in revised form: 7 September 2012 / Accepted: 7 September 2012 / Published: 19 September 2012
(This article belongs to the Section Molecular Recognition)
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Abstract: The aggregation of β-amyloid peptide (Aβ) into fibrils plays an important role in the pathogenesis of Alzheimer’s disease (AD). Metal ions including copper and zinc are closely connected to the precipitation and toxicity of Aβ. In this study, a surface plasmon resonance (SPR) biosensor was constructed to investigate the interactions between Aβ and metal ions. Aβ peptide was immobilized on the SPR chip surface through a preformed alkanethiol self-assembled monolayer (SAM). Our observations indicate that the immobilized Aβ undergoes a conformational change upon exposure to the metal ions. A difference in metal binding affinity between Aβ1–28 and Aβ1–42 was also detected. The results suggest that SPR is an effective method to characterize the interactions between Aβ and metal ions.
Keywords: Alzheimer’s disease; β-amyloid peptide; surface plasmon resonance; metal ions

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MDPI and ACS Style

Yao, F.; Zhang, R.; Tian, H.; Li, X. Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor. Int. J. Mol. Sci. 2012, 13, 11832-11843.

AMA Style

Yao F, Zhang R, Tian H, Li X. Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor. International Journal of Molecular Sciences. 2012; 13(9):11832-11843.

Chicago/Turabian Style

Yao, Fujun; Zhang, Ruiping; Tian, He; Li, Xiangjun. 2012. "Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor." Int. J. Mol. Sci. 13, no. 9: 11832-11843.

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