Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Amino-Functionalized Mesoporous Silica
2.2. Assignment of UV/VIS and FTIR Spectra of Amino-Functionalized Mesoporous Silica after Adsorption Myoglobin
2.3. Evaluation of Peroxidative Activity of Myoglobin
2.4. Antibacterial Property of Lysozyme
3. Experimental Section
3.1. Materials
3.2. Experimental Procedures
3.2.1. Synthesis of Aminopropyl-Functionalized SBA-15 Mesoporous Silica
3.2.2. Characterization of the Amino-Functionalized Mesoporous Silica Materials
3.2.3. Adsorption of Myoglobin
3.2.4. Characterization of Amino-Functionalized Mesoporous Silica Materials after Adsorption
3.2.5. Measurement of Peroxidase Activity after Adsorption
3.2.6. Glutaraldehyde Coupling Procedure
3.2.7. Enzymatic Activity Assay
3.2.7.1. Assay of Myoglobin
3.2.7.2. Assay of Lysozyme
4. Conclusions
Acknowledgments
References
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Myoglobin | Lysozyme | |
---|---|---|
Protein loading (mg/g) | 88.5 mg/g | 122.4 mg/g |
Enzyme activity (U/g) | 1398 U/g | 1.32 × 106 U/g |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Dong, M.; Wu, Z.; Lu, M.; Wang, Z.; Li, Z. Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor. Int. J. Mol. Sci. 2012, 13, 11443-11454. https://doi.org/10.3390/ijms130911443
Dong M, Wu Z, Lu M, Wang Z, Li Z. Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor. International Journal of Molecular Sciences. 2012; 13(9):11443-11454. https://doi.org/10.3390/ijms130911443
Chicago/Turabian StyleDong, Mengxing, Zhuofu Wu, Ming Lu, Zhi Wang, and Zhengqiang Li. 2012. "Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor" International Journal of Molecular Sciences 13, no. 9: 11443-11454. https://doi.org/10.3390/ijms130911443