Isolation and Characterization of 13 Microsatellite Loci from a Korean Endemic Species, Sophora koreensis (Fabaceae)
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Isolation of Microsatellite Markers
3.2. Primer Validation
4. Conclusions
Acknowledgments
References
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Locus | Mt. Bibong (n = 30) | Hanjeon-ri (n = 10) | ||||||
---|---|---|---|---|---|---|---|---|
Na | Ho | He | p-value | Na | Ho | He | p-value | |
Skor1 | 4 | 0.733 | 0.642 | 0.043 | 3 | 0.800 | 0.556 | 0.367 |
Skor2 | 8 | 0.768 | 0.783 | 0.036 | 4 | 0.600 | 0.611 | 0.213 |
Skor3 | 4 | 0.367 | 0.375 | 0.495 | 3 | 0.800 | 0.556 | 0.368 |
Skor4 | 4 | 0.700 | 0.544 | 0.092 | 3 | 0.800 | 0.644 | 0.834 |
Skor5 | 4 | 0.700 | 0.578 | 0.176 | 2 | 0.800 | 0.489 | 0.176 |
Skor6 | 9 | 0.667 | 0.602 | 0.378 | 5 | 1.000 | 0.744 | 0.004* |
Skor7 | 7 | 0.900 | 0.807 | 0.000* | 4 | 1.000 | 0.744 | 0.291 |
Skor8 | 7 | 0.867 | 0.795 | 0.230 | 5 | 0.800 | 0.800 | 0.010 |
Skor9 | 7 | 0.533 | 0.726 | 0.010 | 6 | 0.800 | 0.717 | 0.133 |
Skor10 | 13 | 0.933 | 0.864 | 0.000* | 5 | 0.800 | 0.761 | 0.003* |
Skor11 | 7 | 0.533 | 0.638 | 0.147 | 3 | 0.200 | 0.367 | 0.007 |
Skor12 | 4 | 0.267 | 0.571 | 0.000* | 2 | 0.200 | 0.189 | 1.000 |
Skor13 | 6 | 0.467 | 0.699 | 0.000* | 4 | 0.400 | 0.661 | 0.044 |
Locus | Primer sequences (5′→3′) | Repeat motif | Size range (bp) | GenBank Accession No. |
---|---|---|---|---|
Skor1 | F: ACTCAAAGCTGGAAGGGT R: TTCCGGTTGTTCGAGTCT | (TG)13 | 251–273 | JX131650 |
Skor2 | F: TGAACTACTACTCCGCTT R: ACCCCGTCAGCCAATACTTA | (AG)14 | 266–292 | JX131651 |
Skor3 | F: ATTCTGACTATTGGGCTAGTG R: ATCTGCTAAACCCTTGCTG | (GT)20 | 222–244 | JX131652 |
Skor4 | F: TCAATCACTATAAGGGTTCAG R: AAGTGTCAGGTGCATAAACC | (CAA)12 | 240–252 | JX131653 |
Skor5 | F: ATCAACTACTTCATGCGC R: ACAATCACTCCCAGCATAC | (TG)14 | 260–276 | JX131654 |
Skor6 | F: ACAAAAAGAAATGGTCGCG R: ACATGCCCCTCTGCCCCAAC | (CA)21(GA)10 | 242–294 | JX131655 |
Skor7 | F: TTGAAAAGTCCCACCTAGC R: TATCACCGCCAAGAACTTG | (GA)26 | 273–291 | JX131656 |
Skor8 | F: TCTAGGAGGAAATAGGGAG R: TAGACTCTTGGAACAAGAAC | (CA)12 | 285–297 | JX131657 |
Skor9 | F: TGGACACCCCTTATGGCTG R: ACCAAAGATTCCCGTGACAC | (AG)19 | 248–278 | JX131658 |
Skor10 | F: TTGGGATAGAGTCACGTCC R: ACCGAACATGTCTCATTACC | (GT)11(GA)15 | 222–270 | JX131659 |
Skor11 | F: TAAAGTGCATGTTCCCAG R: TGCTGAACCCTTAGAGAGG | (CA)10 | 256–296 | JX131660 |
Skor12 | F: TGAACTTAGCATTCCATTC R: AAGCGGTTAGACTTTCAGC | (GA)10 | 253–267 | JX131661 |
Skor13 | F: TTCAATGGGTTATTGGTCC R: ATGTATCTTCTGGGACTCG | (TG)14 | 238–250 | JX131662 |
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Lee, J.-Y.; Lee, D.-H.; Choi, B.-H. Isolation and Characterization of 13 Microsatellite Loci from a Korean Endemic Species, Sophora koreensis (Fabaceae). Int. J. Mol. Sci. 2012, 13, 10765-10770. https://doi.org/10.3390/ijms130910765
Lee J-Y, Lee D-H, Choi B-H. Isolation and Characterization of 13 Microsatellite Loci from a Korean Endemic Species, Sophora koreensis (Fabaceae). International Journal of Molecular Sciences. 2012; 13(9):10765-10770. https://doi.org/10.3390/ijms130910765
Chicago/Turabian StyleLee, Ji-Yeon, Dong-Hyuk Lee, and Byoung-Hee Choi. 2012. "Isolation and Characterization of 13 Microsatellite Loci from a Korean Endemic Species, Sophora koreensis (Fabaceae)" International Journal of Molecular Sciences 13, no. 9: 10765-10770. https://doi.org/10.3390/ijms130910765