Int. J. Mol. Sci. 2012, 13(8), 9609-9614; doi:10.3390/ijms13089609
Article

Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae)

Received: 2 May 2012; in revised form: 18 July 2012 / Accepted: 25 July 2012 / Published: 2 August 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Clivia is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. In order to assess the phylogenetic relationships and genetic diversity of the wild Clivia species and cultivars, we isolated AC-enriched repeats using FIASCO from a single clone each of C. miniata Regel. and Clivia nobilis Lindl. Of the fourteen repeats, 10 were polymorphic and 4 were monomorphic. The polymorphic marker loci were characterized using 61 Clivia accessions. The number of alleles ranged from two to six, observed heterozygosity ranged from 0.04 to 1.00 and expected heterozygosity ranged from 0.04 to 0.83. These microsatellite marker loci provide tools for future studies of Clivia species and cultivars.
Keywords: Clivia miniata; Clivia nobilis; genetics diversity; microsatellite
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MDPI and ACS Style

Gao, W.; Zhao, H.; Liu, Y.; Li, M.-R.; Nurmamat, E.; Li, L.-F.; Ren, Y.-Y.; Xiao, H.-X. Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae). Int. J. Mol. Sci. 2012, 13, 9609-9614.

AMA Style

Gao W, Zhao H, Liu Y, Li M-R, Nurmamat E, Li L-F, Ren Y-Y, Xiao H-X. Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae). International Journal of Molecular Sciences. 2012; 13(8):9609-9614.

Chicago/Turabian Style

Gao, Wei; Zhao, He; Liu, Ying; Li, Ming-Rui; Nurmamat, Eliyas; Li, Lin-Feng; Ren, Yue-Ying; Xiao, Hong-Xing. 2012. "Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae)." Int. J. Mol. Sci. 13, no. 8: 9609-9614.

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