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Int. J. Mol. Sci. 2012, 13(8), 9609-9614; doi:10.3390/ijms13089609

Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae)

1
College of Traditional Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China
2
College of Biological Science and Technology, Changchun University, Changchun 130012, China
3
Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China
4
College of Chemistry and Life Sciences, Yili Normal University, Yili, 835000, China
*
Authors to whom correspondence should be addressed.
Received: 2 May 2012 / Revised: 18 July 2012 / Accepted: 25 July 2012 / Published: 2 August 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Clivia is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. In order to assess the phylogenetic relationships and genetic diversity of the wild Clivia species and cultivars, we isolated AC-enriched repeats using FIASCO from a single clone each of C. miniata Regel. and Clivia nobilis Lindl. Of the fourteen repeats, 10 were polymorphic and 4 were monomorphic. The polymorphic marker loci were characterized using 61 Clivia accessions. The number of alleles ranged from two to six, observed heterozygosity ranged from 0.04 to 1.00 and expected heterozygosity ranged from 0.04 to 0.83. These microsatellite marker loci provide tools for future studies of Clivia species and cultivars.
Keywords: Clivia miniata; Clivia nobilis; genetics diversity; microsatellite Clivia miniata; Clivia nobilis; genetics diversity; microsatellite
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Gao, W.; Zhao, H.; Liu, Y.; Li, M.-R.; Nurmamat, E.; Li, L.-F.; Ren, Y.-Y.; Xiao, H.-X. Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae). Int. J. Mol. Sci. 2012, 13, 9609-9614.

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