Int. J. Mol. Sci. 2012, 13(8), 9584-9598; doi:10.3390/ijms13089584
Article

Comparative Studies on the Induction of Trichoderma harzianum Mutanase by α-(1→3)-Glucan-Rich Fruiting Bodies and Mycelia of Laetiporus sulphureus

Adrian Wiater 1,* email, Małgorzata Pleszczyńska 1 email, Janusz Szczodrak 1 email and Grzegorz Janusz 2 email
1 Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland 2 Department of Biochemistry, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
* Author to whom correspondence should be addressed.
Received: 10 July 2012; in revised form: 24 July 2012 / Accepted: 24 July 2012 / Published: 31 July 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Abstract: Mutanase (α-(1→3)-glucanase) is a little-known inductive enzyme that is potentially useful in dentistry. Here, it was shown that the cell wall preparation (CWP) obtained from the fruiting body or vegetative mycelium of polypore fungus Laetiporus sulphureus is rich in α-(1→3)-glucan and can be successfully used for mutanase induction in Trichoderma harzianum. The content of this biopolymer in the CWP depended on the age of fruiting bodies and increased along with their maturation. In the case of CWP prepared from vegetative mycelia, the amount of α-(1→3)-glucan depended on the mycelium age and also on the kind of medium used for its cultivation. All CWPs prepared from the individually harvested fruiting body specimens induced high mutanase activity (0.53–0.82 U/mL) in T. harzianum after 3 days of cultivation. As for the CWPs obtained from the hyphal mycelia of L. sulpureus, the maximal enzyme productivity (0.34 U/mL after 3 days of incubation) was recorded for CWP prepared from the 3 week-old mycelium cultivated in Sabouraud medium. Statistically, a high positive correlation was found between the total percentage content of α-(1→3)-glucan in the CWP and the mutanase activity.
Keywords: Laetiporus sulphureus; mutanase; Trichoderma harzianum; α-(1→3)-glucan; fruiting body; polypore fungus

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MDPI and ACS Style

Wiater, A.; Pleszczyńska, M.; Szczodrak, J.; Janusz, G. Comparative Studies on the Induction of Trichoderma harzianum Mutanase by α-(1→3)-Glucan-Rich Fruiting Bodies and Mycelia of Laetiporus sulphureus. Int. J. Mol. Sci. 2012, 13, 9584-9598.

AMA Style

Wiater A, Pleszczyńska M, Szczodrak J, Janusz G. Comparative Studies on the Induction of Trichoderma harzianum Mutanase by α-(1→3)-Glucan-Rich Fruiting Bodies and Mycelia of Laetiporus sulphureus. International Journal of Molecular Sciences. 2012; 13(8):9584-9598.

Chicago/Turabian Style

Wiater, Adrian; Pleszczyńska, Małgorzata; Szczodrak, Janusz; Janusz, Grzegorz. 2012. "Comparative Studies on the Induction of Trichoderma harzianum Mutanase by α-(1→3)-Glucan-Rich Fruiting Bodies and Mycelia of Laetiporus sulphureus." Int. J. Mol. Sci. 13, no. 8: 9584-9598.

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