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Enzymatic Properties and Mutational Studies of Chalcone Synthase from Physcomitrella patens
Faculty of Biotechnology and Biomolecular Sciences, Enzyme and Microbial Technology Research Group, Universiti Putra Malaysia, Selangor 43400, Malaysia
Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Malaysia
* Author to whom correspondence should be addressed.
Received: 2 May 2012; in revised form: 6 July 2012 / Accepted: 9 July 2012 / Published: 3 August 2012
Abstract: PpCHS is a member of the type III polyketide synthase family and catalyses the synthesis of the flavonoid precursor naringenin chalcone from p-coumaroyl-CoA. Recent research reports the production of pyrone derivatives using either hexanoyl-CoA or butyryl-CoA as starter molecule. The Cys-His-Asn catalytic triad found in other plant chalcone synthase predicted polypeptides is conserved in PpCHS. Site directed mutagenesis involving these amino acids residing in the active-site cavity revealed that the cavity volume of the active-site plays a significant role in the selection of starter molecules as well as product formation. Substitutions of Cys 170 with Arg and Ser amino acids decreased the ability of the PpCHS to utilize hexanoyl-CoA as a starter molecule, which directly effected the production of pyrone derivatives (products). These substitutions are believed to have a restricted number of elongations of the growing polypeptide chain due to the smaller cavity volume of the mutant’s active site.
Keywords: chalcone synthase; site-directed mutagenesis; active site; by-products
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Raja Abdul Rahman, R.N.Z.; Zakaria, I.I.; Salleh, A.B.; Basri, M. Enzymatic Properties and Mutational Studies of Chalcone Synthase from Physcomitrella patens. Int. J. Mol. Sci. 2012, 13, 9673-9691.
Raja Abdul Rahman RNZ, Zakaria II, Salleh AB, Basri M. Enzymatic Properties and Mutational Studies of Chalcone Synthase from Physcomitrella patens. International Journal of Molecular Sciences. 2012; 13(8):9673-9691.
Raja Abdul Rahman, Raja Noor Zaliha; Zakaria, Iffah Izzati; Salleh, Abu Bakar; Basri, Mahiran. 2012. "Enzymatic Properties and Mutational Studies of Chalcone Synthase from Physcomitrella patens." Int. J. Mol. Sci. 13, no. 8: 9673-9691.