Isolation and Characterization of 46 Novel Polymorphic EST-Simple Sequence Repeats (SSR) Markers in Two Sinipercine Fishes (Siniperca) and Cross-Species Amplification
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
4. Conclusions
Acknowledgments
References
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Locus | Accession number | Repeat motif | Primer sequence(5′-3′) | Size range (bp) | Ta (°C) | Na | HO | HE | PIC | p-Value |
---|---|---|---|---|---|---|---|---|---|---|
Sin109 | JQ804765 | (AG)15 | F: GGACACTGGACACTCAAACAT | 220–270 | 54.5 | 4 | 0.2500 | 0.6339 | 0.5747 | 1.0000 |
R: AGAGGATCAAAATTGTGCTTGAA | 246–285 | 54.5 | 6 | 0.6875 | 0.8170 | 0.7756 | 0.9510 | |||
Sin110 | JQ804766 | (AC)15 | F: TGCTGTTTCCTCAAAACCCCT | 177–244 | 54.5 | 6 | 0.7188 | 0.8194 | 0.7798 | 0.9933 |
R: AATCCAAGTGACAGGACGCC | — | — | — | — | — | — | — | |||
Sin112 | JQ804768 | (AC)15 | F: ATCGGCACCTGAGGCAAAAG | 132–166 | 54.5 | 6 | 0.9688 | 0.7897 | 0.7444 | 0.0030 |
R: GCCATCCATAGAGCCACGTC | 129–198 | 54.5 | 9 | 1.0000 | 0.8770 | 0.8479 | 0.0090 | |||
Sin113 | JQ804769 | (TG)15 | F: TCCCCATATCTGCCCTGACC | 90–126 | 54.5 | 6 | 0.8125 | 0.7907 | 0.7439 | 0.9514 |
R: GTGCACATGTCGAGTCAGTA | — | — | — | — | — | — | — | |||
Sin114 | JQ804770 | (AC)14 | F: AAGAGACAAGACACCACCGC | 185–209 | 54.5 | 5 | 0.4375 | 0.6736 | 0.6012 | 0.9347 |
R: ATGGTTTGACGGGAGACAGC | 194–243 | 54.5 | 7 | 1.0000 | 0.8418 | 0.8064 | 0.0039 | |||
Sin116 | JQ804771 | (TG)14(AG)7 | F: ACAATCCCAGCCCTCCTTCT | 212–265 | 54.5 | 6 | 0.5625 | 0.8219 | 0.7813 | 0.9999 |
R: GCAAGGTCCCTTTACATGCAG | 219–259 | 54.5 | 5 | 0.8750 | 0.7877 | 0.7393 | 0.0798 | |||
Sin117 | JQ804772 | (GT)14 | F: GGGCGGAAGACCAACTATGT | 268–291 | 54.5 | 3 | 0.4062 | 0.5332 | 0.4697 | 0.9871 |
R: TTTCTGTCCTTTTTCCTCTCGC | — | — | — | — | — | — | — | |||
Sin118 | JQ804773 | (GT)14 | F: AGGCCACACTTTAGTCACATC | 163–192 | 54.5 | 4 | 0.8750 | 0.7376 | 0.6754 | 0.0393 |
R: ACCACACTCCAGCATTTCCC | 157–189 | 54.5 | 3 | 1.0000 | 0.6225 | 0.5378 | 0.0000 * | |||
Sin119 | JQ804774 | (CA)14 | F: AACAACTTTTTACCGCCAGCC | 180–226 | 54.5 | 4 | 0.8438 | 0.7282 | 0.6652 | 0.1123 |
R: ACCTCTGCTGCACAGCTAATC | — | — | — | — | — | — | — | |||
Sin120 | JQ804775 | (TTTG)7 | F: CCATCCCTCCGACCTTCAGT | 119–134 | 54.5 | 4 | 0.5312 | 0.6900 | 0.6209 | 0.9418 |
R: TTTAGGAACCCGACTCCGCT | — | — | — | — | — | — | — | |||
Sin122 | JQ804777 | (TG)14TAG(GT)7 | F: TGCACTCACACACCTGTCTC | — | — | — | — | — | — | — |
R: AGCAGGATGCTTCATGCACTT | 205–246 | 54.5 | 5 | 1.0000 | 0.6667 | 0.5927 | 0.0000 * | |||
Sin123 | JQ804778 | (AC)14 | F: GATGGTGGTGAAACACTGGCT | 249–307 | 54.5 | 6 | 0.7812 | 0.7773 | 0.7352 | 0.7478 |
R: GTGTTGAGAGGGTCCTGGTG | 198–214 | 56.0 | 3 | 0.5000 | 0.5397 | 0.4683 | 0.9912 | |||
Sin124 | JQ804779 | (CA)14 | F: TCAAACACCACCCACCCCTG | 248–281 | 54.5 | 4 | 0.8750 | 0.7297 | 0.6667 | 0.0180 |
R: ACCGGGACAGGATGGGAGTC | — | — | — | — | — | — | — | |||
Sin125 | JQ804780 | (CA)14 | F: ACCCTCTGTGTGGCGAATGT | 277–311 | 54.5 | 3 | 0.6250 | 0.6265 | 0.5474 | 0.5368 |
R: CGGGACAGGATGGGAGTCG | — | — | — | — | — | — | — | |||
Sin127 | JQ804782 | (TG)14 | F: AGACGTAGCCCAGGCTCAAA | 215–251 | 54.5 | 3 | 0.5938 | 0.5055 | 0.4213 | 0.0303 |
R: TGTGGGGTTCACTACAGGGT | — | — | — | — | — | — | — | |||
Sin128 | JQ804783 | (AC)14 | F: CTGTGCCTCAGTGTGCTGC | 225–257 | 54.5 | 3 | 0.4062 | 0.6394 | 0.5572 | 0.9995 |
R: ACTTGTAATGGGCAAATTGTCACT | — | — | — | — | — | — | — | |||
Sin129 | JQ804784 | (CA)14 | F: ACGCTGCGAGGTGTGATATG | 131–164 | 54.5 | 5 | 0.6250 | 0.7500 | 0.6971 | 0.9857 |
R: CTGGCCCTCGTTAGTGCTTG | 185–217 | 54.5 | 8 | 1.0000 | 0.8457 | 0.8103 | 0.0046 | |||
Sin130 | JQ804785 | (GTGA)7N7(TG)8 | F: CTCGCAGGCTTTTCTCTGCT | 282–300 | 54.5 | 2 | 0.3438 | 0.3963 | 0.3140 | 0.8902 |
R: AGCCATCAGTTCTGTTCTTTCTT | 252–280 | 54.5 | 5 | 0.7188 | 0.7485 | 0.6982 | 0.3062 | |||
Sin131 | JQ804785 | (ATGG)7 | F: GGAGGAAAATAATTTCATTTGGGAT | 180–200 | 54.5 | 3 | 0.1250 | 0.4107 | 0.3665 | 0.9998 |
R: GTCATTGCATTCAAAAGTTAGGCT | — | — | — | — | — | — | — | |||
Sin134 | JQ804789 | (TG)14 | F: GCCCCCTTCTCAACCCACTA | 106–120 | 54.5 | 6 | 1.0000 | 0.8075 | 0.7645 | 0.0008 * |
R: TGCTTTCCAAAGCGAACCGT | 108–134 | 54.5 | 8 | 0.9688 | 0.8621 | 0.8299 | 0.0451 | |||
Sin135 | JQ804790 | (TG)14 | F: GTGATATCTCCTCCTGACGGC | 273–302 | 54.5 | 4 | 0.5938 | 0.5585 | 0.4900 | 0.3484 |
R: ACATTCTGAATTGCAAAGGCTCA | — | — | — | — | — | — | — | |||
Sin136 | JQ804791 | (TG)14 | F: AACTGAAATGTGTGGTGAACTGA | 138–164 | 56.8 | 5 | 0.5000 | 0.7202 | 0.6585 | 0.9243 |
R: GTGTCTCCCAACAAGTGGCA | — | — | — | — | — | — | — | |||
Sin137 | JQ804792 | (TCA)9 | F: AGCGTCTACTGAGGGTCAAACT | 234–280 | 54.5 | 5 | 0.4688 | 0.7207 | 0.6700 | 0.9960 |
R: GGTGGACTGACCAGCAAGGA | — | — | — | — | — | — | — | |||
Sin138 | JQ804793 | (ATC)9 | F: TCATCTGAGGACGACTCGCT | 229–253 | 52.5 | 3 | 0.4375 | 0.5997 | 0.5025 | 0.9772 |
R: AACTTAACTTCCTGCTGTCCCT | — | — | — | — | — | — | — | |||
Sin139 | JQ804794 | (CTC)9 | F: GTGACTGCATCCAGGTGTCG | — | — | — | — | — | — | — |
R: GGCCGAGGTCGGTTGTTATC | 189–207 | 54.5 | 3 | 0.1875 | 0.2803 | 0.2584 | 0.9947 | |||
Sin140 | JQ804795 | (TCA)9 | F: TGTGGTTCTCCTCTCCCACA | 253–304 | 53.2 | 5 | 0.4375 | 0.7336 | 0.6759 | 0.9985 |
R: AGAGGTTGGTGCAGGAGACTT | — | — | — | — | — | — | — | |||
Sin142 | JQ804797 | (CTT)9 | F: CATCAACGCAATGCAAGGGT | 150–180 | 54.5 | 2 | 0.1562 | 0.3289 | 0.2713 | 0.9997 |
R: CTGGAGCCGGACTTGAGGAA | 181–226 | 54.5 | 6 | 0.3438 | 0.6994 | 0.6492 | 0.9998 | |||
Sin143 | JQ804797 | (GTT)7 | F: AAAGCAGGCCAAACAACACC | 198–246 | 54.5 | 5 | 0.4062 | 0.7733 | 0.7206 | 1.0000 |
R: AGGACGGGGAGGCTTTTGAT | — | — | — | — | — | — | — | |||
Sin146 | JQ804800 | (GAG)6 N5 (AAG)9 | F: GTAATCGACACGGACAGCGA | 370–452 | 54.5 | 4 | 0.5938 | 0.6781 | 0.6069 | 0.8240 |
R: CACACACATTCTCCTCAGCGT | — | — | — | — | — | — | — | |||
Sin147 | JQ804801 | (TCC)9 | F: AGATCAGACACCAGGAGGACC | 174–232 | 53.5 | 5 | 0.3125 | 0.7242 | 0.6576 | 0.9971 |
R: AAGACGGAGGCAAAGAACGAC | 192–225 | 54.5 | 4 | 0.5625 | 0.7614 | 0.7026 | 0.9964 | |||
Sin148 | JQ804802 | (CAT)9 | F: CGAGGCCAGGAGTGAACCAA | 255–303 | 53.5 | 3 | 0.6875 | 0.4866 | 0.4009 | 0.0027 |
R: GCACAGCTGGAGGTGTTTCG | — | — | — | — | — | — | — | |||
Sin151 | JQ804805 | (GT)13 | F: GTGCAAGGCCTTAGTCTCTCC | 170–221 | 55.5 | 4 | 0.6875 | 0.6434 | 0.5761 | 0.8897 |
R: GCCCACCAGATCTACCGAGT | — | — | — | — | — | — | — | |||
Sin152 | JQ804806 | (AG)8A(AG)13 | F: TGCGCCACTTTACTGATGGG | 173–242 | 53.5 | 6 | 0.6562 | 0.8105 | 0.7677 | 0.9164 |
R: GCATTAACCAAACCCCGCGA | 185–240 | 54.5 | 8 | 0.9688 | 0.8522 | 0.8193 | 0.1740 | |||
Sin153 | JQ804807 | (AG)13 | F: GCACAGGTTTTTCTAAACATTGCT | 155–208 | 53.5 | 5 | 0.1875 | 0.3795 | 0.3538 | 0.9662 |
R: TGTTGTTATTGTCAGTGTGTTTTCT | 177–215 | 54.5 | 4 | 0.2500 | 0.6806 | 0.6160 | 1.0000 | |||
Sin154 | JQ804808 | (GT)13 | F: ACTGGTTTGTGGTTTGGAGGT | 211–229 | 53.5 | 2 | 0.5625 | 0.4107 | 0.3225 | 0.0356 |
R: ATGATTTTTCTTGCCTTCGTGT | — | — | — | — | — | — | — | |||
Sin155 | JQ804809 | (AC)13 | F: GAATGGTGTGTTGCACAGCG | 157–190 | 53.5 | 3 | 0.2188 | 0.3894 | 0.3473 | 0.9939 |
R: CATTCTAGCATGTGCGAGGC | 160–201 | 54.5 | 7 | 0.6875 | 0.8021 | 0.7600 | 0.9004 | |||
Sin156 | JQ804810 | (AC)13 | F: TAGGAGGCTTTACAACCGGC | 188–205 | 53.5 | 2 | 0.5625 | 0.4107 | 0.3225 | 0.0344 |
R: ATGACCAGCCTCAGGTGTCT | — | — | — | — | — | — | — | |||
Sin157 | JQ804811 | (AC)13 | F: CATTTGCTGGCTCTCACACC | 184–215 | 53.5 | 3 | 0.5000 | 0.4330 | 0.3477 | 0.2070 |
R: TGTTTAATTCATGCCTAGGTTTAGT | — | — | — | — | — | — | — | |||
Sin158 | JQ804812 | (CA)13 | F: TGAGAACTGCCTGAGCCGAG | — | — | — | — | — | — | — |
R: CTGCAGAGCCGTGGAGACTA | 210–248 | 54.5 | 3 | 0.9062 | 0.5303 | 0.4145 | 0.0000 * | |||
Sin159 | JQ804813 | (TG)13 | F: CGCTGATCGCTCTGTGCTCCC | 196–234 | 53.5 | 5 | 0.6875 | 0.7614 | 0.7108 | 0.7259 |
R: ACACGGAAGCTGGTGAGCGG | 199–233 | 56.0 | 5 | 0.9688 | 0.6379 | 0.5682 | 0.0000 * | |||
Sin160 | JQ804814 | (TG)13 | F: CCACTGGAGCCCACATGGCA | 307–360 | 55.5 | 5 | 0.8125 | 0.6711 | 0.6123 | 0.0151 |
R: TGAGTGGGCGCTACTGTGTGT | 291–331 | 54.5 | 5 | 0.7812 | 0.7659 | 0.7137 | 0.5928 | |||
Sin162 | JQ804815 | (TA)13 | F: TGCTTTGCTGGTTGGCAGGCT | 294–368 | 53.5 | 5 | 0.1875 | 0.6270 | 0.5651 | 1.0000 |
R: CGTGGAGGTGCGACGCGTAA | — | — | — | — | — | — | — | |||
Sin163 | JQ804817 | (CA)13 | F: ACAGCCAGGCTCCTCCACCT | 230–269 | 53.5 | 8 | 0.6875 | 0.8482 | 0.8134 | 0.9597 |
R: TCTTTCACAGGCAAACCACTGCT | 225–273 | 53.5 | 6 | 0.4688 | 0.7803 | 0.7392 | 1.0000 | |||
Sin166 | JQ804819 | (GA)13 | F: GAAATTGAAGAAGACAAGGTGATG | 204–231 | 53.5 | 3 | 0.2500 | 0.4504 | 0.4012 | 0.9998 |
R: CTGCTTTTGGCAGGAGCTAA | — | — | — | — | — | — | — | |||
Sin169 | JQ804822 | (AC)13 | F: TGACAAATCACTGGGTTTACTCCT | 214–284 | 53.5 | 5 | 0.5625 | 0.6443 | 0.5790 | 0.9164 |
R: GACATGCTGCTCTCCGATCC | — | — | — | — | — | — | — | |||
Sin170 | JQ804823 | (GT)13 | F: CTTGAGTGGTTGATTGTGCCCT | 242–270 | 55.5 | 4 | 0.7188 | 0.5491 | 0.4990 | 0.0015 |
R: GCAGACATTGCTGAGGGATGAA | — | — | — | — | — | — | — |
Species | ||||
---|---|---|---|---|
Locus | S. undulate | S. obscura | S. kneri | C. whiteheadi |
Sin109 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin110 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin112 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin113 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin114 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin116 | 54.5 | 54.5 | 54.5 | — |
Sin117 | 54.5 | 54.5 | 54.5 | — |
Sin118 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin119 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin120 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin122 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin123 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin124 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin125 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin127 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin128 | 54.5 | 54.5 | 54.5 | — |
Sin129 | 54.5 | 54.5 | — | 54.5 |
Sin130 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin131 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin134 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin135 | 54.5 | 54.5 | 54.5 | — |
Sin136 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin137 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin138 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin139 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin140 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin142 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin143 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin146 | 54.5 | 54.5 | 54.5 | — |
Sin147 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin148 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin151 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin152 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin153 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin154 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin155 | — | — | — | — |
Sin156 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin157 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin158 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin159 | 54.5 | 54.5 | 54.5 | 52.8 |
Sin160 | 54.5 | 54.5 | 54.5 | — |
Sin162 | 54.5 | 54.5 | 57.0 | 51.1 |
Sin163 | 54.5 | 54.5 | 54.5 | 52.8 |
Sin166 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin169 | 54.5 | 54.5 | 54.5 | 54.5 |
Sin170 | 54.5 | 54.5 | 54.5 | 54.5 |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Qu, C.; Liang, X.; Huang, W.; Cao, L. Isolation and Characterization of 46 Novel Polymorphic EST-Simple Sequence Repeats (SSR) Markers in Two Sinipercine Fishes (Siniperca) and Cross-Species Amplification. Int. J. Mol. Sci. 2012, 13, 9534-9544. https://doi.org/10.3390/ijms13089534
Qu C, Liang X, Huang W, Cao L. Isolation and Characterization of 46 Novel Polymorphic EST-Simple Sequence Repeats (SSR) Markers in Two Sinipercine Fishes (Siniperca) and Cross-Species Amplification. International Journal of Molecular Sciences. 2012; 13(8):9534-9544. https://doi.org/10.3390/ijms13089534
Chicago/Turabian StyleQu, Chunmei, Xufang Liang, Wei Huang, and Liang Cao. 2012. "Isolation and Characterization of 46 Novel Polymorphic EST-Simple Sequence Repeats (SSR) Markers in Two Sinipercine Fishes (Siniperca) and Cross-Species Amplification" International Journal of Molecular Sciences 13, no. 8: 9534-9544. https://doi.org/10.3390/ijms13089534