Int. J. Mol. Sci. 2012, 13(8), 9400-9418; doi:10.3390/ijms13089400
Article

Early Amyloidogenic Oligomerization Studied through Fluorescence Lifetime Correlation Spectroscopy

1email, 2email, 1email, 2email, 1email, 2,†email and 1,* email
Received: 19 June 2012; in revised form: 13 July 2012 / Accepted: 19 July 2012 / Published: 25 July 2012
(This article belongs to the Special Issue Advances in Single Molecule Spectroscopy)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Amyloidogenic protein aggregation is a persistent biomedical problem. Despite active research in disease-related aggregation, the need for multidisciplinary approaches to the problem is evident. Recent advances in single-molecule fluorescence spectroscopy are valuable for examining heterogenic biomolecular systems. In this work, we have explored the initial stages of amyloidogenic aggregation by employing fluorescence lifetime correlation spectroscopy (FLCS), an advanced modification of conventional fluorescence correlation spectroscopy (FCS) that utilizes time-resolved information. FLCS provides size distributions and kinetics for the oligomer growth of the SH3 domain of α-spectrin, whose N47A mutant forms amyloid fibrils at pH 3.2 and 37 °C in the presence of salt. The combination of FCS with additional fluorescence lifetime information provides an exciting approach to focus on the initial aggregation stages, allowing a better understanding of the fibrillization process, by providing multidimensional information, valuable in combination with other conventional methodologies.
Keywords: amyloids; protein aggregation; pulsed interleaved excitation; protein oligomers; single-molecule fluorescence
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MDPI and ACS Style

Paredes, J.M.; Casares, S.; Ruedas-Rama, M.J.; Fernandez, E.; Castello, F.; Varela, L.; Orte, A. Early Amyloidogenic Oligomerization Studied through Fluorescence Lifetime Correlation Spectroscopy. Int. J. Mol. Sci. 2012, 13, 9400-9418.

AMA Style

Paredes JM, Casares S, Ruedas-Rama MJ, Fernandez E, Castello F, Varela L, Orte A. Early Amyloidogenic Oligomerization Studied through Fluorescence Lifetime Correlation Spectroscopy. International Journal of Molecular Sciences. 2012; 13(8):9400-9418.

Chicago/Turabian Style

Paredes, Jose M.; Casares, Salvador; Ruedas-Rama, Maria J.; Fernandez, Elena; Castello, Fabio; Varela, Lorena; Orte, Angel. 2012. "Early Amyloidogenic Oligomerization Studied through Fluorescence Lifetime Correlation Spectroscopy." Int. J. Mol. Sci. 13, no. 8: 9400-9418.

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