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Int. J. Mol. Sci. 2012, 13(8), 10583-10593; doi:10.3390/ijms130810583

Development of Microsatellite Markers for the Korean Mussel, Mytilus coruscus (Mytilidae) Using Next-Generation Sequencing

Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea
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Received: 5 June 2012 / Revised: 14 August 2012 / Accepted: 16 August 2012 / Published: 22 August 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Mytilus coruscus (family Mytilidae) is one of the most important marine shellfish species in Korea. During the past few decades, this species has become endangered due to the loss of habitats and overfishing. Despite this species’ importance, information on its genetic background is scarce. In this study, we developed microsatellite markers for M. coruscus using next-generation sequencing. A total of 263,900 raw reads were obtained from a quarter-plate run on the 454 GS-FLX titanium platform, and 176,327 unique sequences were generated with an average length of 381 bp; 2569 (1.45%) sequences contained a minimum of five di- to tetra-nucleotide repeat motifs. Of the 51 loci screened, 46 were amplified successfully, and 22 were polymorphic among 30 individuals, with seven of trinucleotide repeats and three of tetranucleotide repeats. All loci exhibited high genetic variability, with an average of 17.32 alleles per locus, and the mean observed and expected heterozygosities were 0.67 and 0.90, respectively. In addition, cross-amplification was tested for all 22 loci in another congener species, M. galloprovincialis. None of the primer pairs resulted in effective amplification, which might be due to their high mutation rates. Our work demonstrated the utility of next-generation 454 sequencing as a method for the rapid and cost-effective identification of microsatellites. The high degree of polymorphism exhibited by the 22 newly developed microsatellites will be useful in future conservation genetic studies of this species. View Full-Text
Keywords: Korean mussel; Mytilus coruscus; microsatellite loci; next-generation sequencing; genetic variability Korean mussel; Mytilus coruscus; microsatellite loci; next-generation sequencing; genetic variability
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

An, H.S.; Lee, J.W. Development of Microsatellite Markers for the Korean Mussel, Mytilus coruscus (Mytilidae) Using Next-Generation Sequencing. Int. J. Mol. Sci. 2012, 13, 10583-10593.

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