Abstract: Elongation factor (EF) is a key regulation factor for translation in many organisms, including plants, bacteria, fungi, animals and insects. To investigate the nature and function of elongation factor 1β' from Spodoptera exigua (SeEF-1β'), its cDNA was cloned. This contained an open reading frame of 672 nucleotides encoding a protein of 223 amino acids with a predicted molecular weight of 24.04 kDa and pI of 4.53. Northern blotting revealed that SeEF-1β' mRNA is expressed in brain, epidermis, fat body, midgut, Malpighian tubules, ovary and tracheae. RT-PCR revealed that SeEF-1β' mRNA is expressed at different levels in fat body and whole body during different developmental stages. In RNAi experiments, the survival rate of insects injected with SeEF-1β' dsRNA was 58.7% at 36 h after injection, which was significantly lower than three control groups. Other elongation factors and transcription factors were also influenced when EF-1β' was suppressed. The results demonstrate that SeEF-1β' is a key gene in transcription in S. exigua.
Keywords: elongation factor; cloning; expression pattern; RNAi; Spodoptera exigua
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Zhao, L.-N.; Qin, Z.; Wei, P.; Guo, H.-S.; Dang, X.-L.; Wang, S.-G.; Tang, B. Elongation Factor 1β' Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference. Int. J. Mol. Sci. 2012, 13, 8126-8141.
Zhao L-N, Qin Z, Wei P, Guo H-S, Dang X-L, Wang S-G, Tang B. Elongation Factor 1β' Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference. International Journal of Molecular Sciences. 2012; 13(7):8126-8141.
Zhao, Li-Na; Qin, Zi; Wei, Ping; Guo, Hong-Shuang; Dang, Xiang-Li; Wang, Shi-Gui; Tang, Bin. 2012. "Elongation Factor 1β' Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference." Int. J. Mol. Sci. 13, no. 7: 8126-8141.