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Elongation Factor 1β' Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference
Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
Zhejiang Institute of Subtropical Crops, Wenzhou, Zhejiang 325005, China
* Author to whom correspondence should be addressed.
Received: 29 April 2012; in revised form: 13 June 2012 / Accepted: 18 June 2012 / Published: 29 June 2012
Abstract: Elongation factor (EF) is a key regulation factor for translation in many organisms, including plants, bacteria, fungi, animals and insects. To investigate the nature and function of elongation factor 1β' from Spodoptera exigua (SeEF-1β'), its cDNA was cloned. This contained an open reading frame of 672 nucleotides encoding a protein of 223 amino acids with a predicted molecular weight of 24.04 kDa and pI of 4.53. Northern blotting revealed that SeEF-1β' mRNA is expressed in brain, epidermis, fat body, midgut, Malpighian tubules, ovary and tracheae. RT-PCR revealed that SeEF-1β' mRNA is expressed at different levels in fat body and whole body during different developmental stages. In RNAi experiments, the survival rate of insects injected with SeEF-1β' dsRNA was 58.7% at 36 h after injection, which was significantly lower than three control groups. Other elongation factors and transcription factors were also influenced when EF-1β' was suppressed. The results demonstrate that SeEF-1β' is a key gene in transcription in S. exigua.
Keywords: elongation factor; cloning; expression pattern; RNAi; Spodoptera exigua
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MDPI and ACS Style
Zhao, L.-N.; Qin, Z.; Wei, P.; Guo, H.-S.; Dang, X.-L.; Wang, S.-G.; Tang, B. Elongation Factor 1β' Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference. Int. J. Mol. Sci. 2012, 13, 8126-8141.
Zhao L-N, Qin Z, Wei P, Guo H-S, Dang X-L, Wang S-G, Tang B. Elongation Factor 1β' Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference. International Journal of Molecular Sciences. 2012; 13(7):8126-8141.
Zhao, Li-Na; Qin, Zi; Wei, Ping; Guo, Hong-Shuang; Dang, Xiang-Li; Wang, Shi-Gui; Tang, Bin. 2012. "Elongation Factor 1β' Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference." Int. J. Mol. Sci. 13, no. 7: 8126-8141.