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Int. J. Mol. Sci. 2012, 13(6), 7762-7775; doi:10.3390/ijms13067762

Preparation of Porous Scaffolds from Silk Fibroin Extracted from the Silk Gland of Bombyx mori (B. mori)

Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China
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Received: 25 April 2012 / Revised: 14 June 2012 / Accepted: 15 June 2012 / Published: 21 June 2012
(This article belongs to the Section Material Sciences and Nanotechnology)
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Abstract

In order to use a simple and ecofriendly method to prepare porous silk scaffolds, aqueous silk fibroin solution (ASF) was extracted from silk gland of 7-day-old fifth instar larvae of Bombyx mori (B. mori). SDS-page analysis indicated that the obtained fibroin had a molecular weight higher than 200 kDa. The fabrication of porous scaffolds from ASF was achieved by using the freeze-drying method. The pore of porous scaffolds is homogenous and tends to become smaller with an increase in the concentration of ASF. Conversely, the porosity is decreased. The porous scaffolds show impressive compressive strength which can be as high as 6.9 ± 0.4 MPa. Furthermore, ASF has high cell adhesion and growth activity. It also exhibits high ALP activity. This implies that porous scaffolds prepared from ASF have biocompatibility. Therefore, the porous scaffolds prepared in this study have potential application in tissue engineering due to the impressive compressive strength and biocompatibility. View Full-Text
Keywords: B. mori fibroin; silk gland; porous scaffolds; compressive strength; cell adhesion and growth activity; ALP activity B. mori fibroin; silk gland; porous scaffolds; compressive strength; cell adhesion and growth activity; ALP activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Yang, M.; Shuai, Y.; He, W.; Min, S.; Zhu, L. Preparation of Porous Scaffolds from Silk Fibroin Extracted from the Silk Gland of Bombyx mori (B. mori). Int. J. Mol. Sci. 2012, 13, 7762-7775.

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