Int. J. Mol. Sci. 2012, 13(5), 6424-6439; doi:10.3390/ijms13056424
Article

CD133-Positive Cells Might Be Responsible for Efficient Proliferation of Human Meningioma Cells

1 Department of Neurosurgery, Huashan Hospital, Fudan University, No.12, Middle wulumuqi road, Shanghai 200040, China 2 Department of Endocrinology, Huashan Hospital, Fudan University, No.12, Middle wulumuqi road, Shanghai 200040, China 3 Department of Neuropathology, Huashan Hospital, Fudan University, No.12, Middle wulumuqi road, Shanghai 200040, China
* Author to whom correspondence should be addressed.
Received: 27 March 2012; in revised form: 20 April 2012 / Accepted: 14 May 2012 / Published: 23 May 2012
PDF Full-text Download PDF Full-Text [852 KB, Updated Version, uploaded 24 May 2012 14:42 CEST]
The original version is still available [853 KB, uploaded 23 May 2012 13:50 CEST]
Abstract: Owing to lack of appropriate model systems, investigations of meningioma biology have come to a stop. In this study, we developed a comprehensive digestion method and defined a culture system. Using this method and system, primary meningioma cells in conditioned suspension medium and a hypoxic environment could be amplified in spheres and were passaged for more than ten generations. Meningioma sphere cells were positive for meningioma cell markers and negative for markers of neural cell types. Importantly, we found the cells expressed the stem cell marker, CD133, but not nestin. All of the tumor sphere cell populations showed a slower degree of cell proliferation than that of human glioma cells and fetal neural stem cells (NSCs). Further studies showed that the proliferative rate was positively correlated with CD133 expression. The higher the CD133 expression, the faster the cell proliferation. With the increase in cell generations, the cell proliferation rate gradually slowed down, and CD133 expression also decreased. Single CD133+ cells rather than CD133 cells could form spheres. Thus, the results above indicated that those cells expressing CD133 in spheres might be stem-like cells, which may be responsible for efficient amplification of human meningioma cells. Decreased expression of CD133 may lead to the failure of long-term passaging.
Keywords: meningioma cells; CD133; sphere; proliferation

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Tang, H.; Gong, Y.; Mao, Y.; Xie, Q.; Zheng, M.; Wang, D.; Zhu, H.; Wang, X.; Chen, H.; Chen, X.; Zhou, L. CD133-Positive Cells Might Be Responsible for Efficient Proliferation of Human Meningioma Cells. Int. J. Mol. Sci. 2012, 13, 6424-6439.

AMA Style

Tang H, Gong Y, Mao Y, Xie Q, Zheng M, Wang D, Zhu H, Wang X, Chen H, Chen X, Zhou L. CD133-Positive Cells Might Be Responsible for Efficient Proliferation of Human Meningioma Cells. International Journal of Molecular Sciences. 2012; 13(5):6424-6439.

Chicago/Turabian Style

Tang, Hailiang; Gong, Ye; Mao, Ying; Xie, Qing; Zheng, Mingzhe; Wang, Daijun; Zhu, Hongda; Wang, Xuanchun; Chen, Hong; Chen, Xiancheng; Zhou, Liangfu. 2012. "CD133-Positive Cells Might Be Responsible for Efficient Proliferation of Human Meningioma Cells." Int. J. Mol. Sci. 13, no. 5: 6424-6439.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert