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Int. J. Mol. Sci. 2012, 13(5), 5844-5850; doi:10.3390/ijms13055844

Isolation and Characterization of 15 New Microsatellite Markers in Oncomelania hupensis, the Snail Intermediate Host of Schistosoma japonicum in Mainland China

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China
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Received: 27 March 2012 / Revised: 19 April 2012 / Accepted: 10 May 2012 / Published: 15 May 2012
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Abstract

Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum, which plays a key role during the transmission of schistosomiasis. It is mainly found in the Yangtze River valley and mountains or hills in southwest China. In this paper, we described 15 new microsatellite makers in O. hupensis. Polymorphism of each locus was assessed in 80 individuals from four wild populations (n = 20 per population). The number of alleles per locus ranged from 6 to 29, with an average of 15.8. The observed (HO) and expected (HE) heterozygosities varied from 0.397 to 0.851 and from 0.696 to 0.948, respectively. These microsatellite markers will be useful for population genetic studies and genome mapping in O. hupensis.
Keywords: Oncomelania hupensis; microsatellites DNA; polymorphism; population genetics Oncomelania hupensis; microsatellites DNA; polymorphism; population genetics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhang, L.; Li, S.; Wang, Q.; Qian, Y.; Liu, Q.; Yang, P.; Zhou, X. Isolation and Characterization of 15 New Microsatellite Markers in Oncomelania hupensis, the Snail Intermediate Host of Schistosoma japonicum in Mainland China. Int. J. Mol. Sci. 2012, 13, 5844-5850.

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