Next Article in Journal
Ginsenoside Rg3 Reduces Lipid Accumulation with AMP-Activated Protein Kinase (AMPK) Activation in HepG2 Cells
Previous Article in Journal
Characterization of CdTe Films Deposited at Various Bath Temperatures and Concentrations Using Electrophoretic Deposition
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 13
Issue 5
10.3390/ijms13055715
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Immune Responses in Pigs Induced by Recombinant DNA Vaccine Co-Expressing Swine IL-18 and Membrane Protein of Porcine Reproductive and Respiratory Syndrome Virus

by
Xiaodong Zhang
,
Xiaoli Wang
,
Lianzhi Mu
and
Zhuang Ding
*
College of Animal Science and Veterinary Medicine, and Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(5), 5715-5728; https://doi.org/10.3390/ijms13055715
Submission received: 21 February 2012 / Revised: 15 April 2012 / Accepted: 3 May 2012 / Published: 11 May 2012
Browse Figures
Versions Notes

Abstract

In this study, two DNA vaccines, which express the membrane (M) protein of porcine respiratory and reproductive syndrome virus (PRRSV) (pEGFP-M) and co-express both M and swine IL-18 (pEGFP-IL18-M), were constructed and their abilities to induce humoral and cellular responses in piglets were comparatively evaluated. Experimental results showed that both recombinant DNA vaccines could not elicit neutralizing antibodies in the immunized piglets. However, both DNA vaccines elicited Th1-biased cellular immune responses. Notably, pigs immunized with the plasmid pEGFP-IL18-M developed significantly higher levels of IFN-γ and IL-2 production response and stronger specific T-lymphocyte proliferation response than the pigs inoculated with the plasmids pEGFP-M and pEGFP-IL18 (P < 0.05). These results illustrated that co-expression of M and IL-18 proteins could significantly improve the potency of DNA vaccination on the activation of vaccine-induced virus-specific cell-mediated immune responses in pigs, which may be used as a strategy to develop a new generation of vaccines against highly pathogenic PRRSV.
Keywords: PRRS; M protein; IL-18; immune response; DNA vaccine PRRS; M protein; IL-18; immune response; DNA vaccine

Share and Cite

MDPI and ACS Style

Zhang, X.; Wang, X.; Mu, L.; Ding, Z. Immune Responses in Pigs Induced by Recombinant DNA Vaccine Co-Expressing Swine IL-18 and Membrane Protein of Porcine Reproductive and Respiratory Syndrome Virus. Int. J. Mol. Sci. 2012, 13, 5715-5728. https://doi.org/10.3390/ijms13055715

AMA Style

Zhang X, Wang X, Mu L, Ding Z. Immune Responses in Pigs Induced by Recombinant DNA Vaccine Co-Expressing Swine IL-18 and Membrane Protein of Porcine Reproductive and Respiratory Syndrome Virus. International Journal of Molecular Sciences. 2012; 13(5):5715-5728. https://doi.org/10.3390/ijms13055715

Chicago/Turabian Style

Zhang, Xiaodong, Xiaoli Wang, Lianzhi Mu, and Zhuang Ding. 2012. "Immune Responses in Pigs Induced by Recombinant DNA Vaccine Co-Expressing Swine IL-18 and Membrane Protein of Porcine Reproductive and Respiratory Syndrome Virus" International Journal of Molecular Sciences 13, no. 5: 5715-5728. https://doi.org/10.3390/ijms13055715

APA Style

Zhang, X., Wang, X., Mu, L., & Ding, Z. (2012). Immune Responses in Pigs Induced by Recombinant DNA Vaccine Co-Expressing Swine IL-18 and Membrane Protein of Porcine Reproductive and Respiratory Syndrome Virus. International Journal of Molecular Sciences, 13(5), 5715-5728. https://doi.org/10.3390/ijms13055715

Article Metrics

Back to TopTop