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Novel Drug Delivery System Based on Docetaxel-Loaded Nanocapsules as a Therapeutic Strategy Against Breast Cancer Cells
Biocolloid and Fluid Physics Group, Department of Applied Physics, University of Granada, 18071 Granada, Spain
Human Anatomy and Embryology Department, Regenerative Biomedicine Institute (IBIMER), Campus de la Salud, University of Granada, 18071 Granada, Spain
Department of Applied Physics II, University of Malaga, 29071 Malaga, Spain
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 8 February 2012; in revised form: 7 March 2012 / Accepted: 6 April 2012 / Published: 19 April 2012
Abstract: In the field of cancer therapy, lipid nanocapsules based on a core-shell structure are promising vehicles for the delivery of hydrophobic drugs such as docetaxel. The main aim of this work was to evaluate whether docetaxel-loaded lipid nanocapsules improved the anti-tumor effect of free docetaxel in breast cancer cells. Three docetaxel-loaded lipid nanocapsules were synthesized by solvent displacement method. Cytotoxic assays were evaluated in breast carcinoma (MCF-7) cells treated by the sulforhodamine B colorimetric method. Cell cycle was studied by flow cytometry and Annexin V-FITC, and apoptosis was evaluated by using propidium iodide assays. The anti-proliferative effect of docetaxel appeared much earlier when the drug was encapsulated in lipid nanoparticles than when it was free. Docetaxel-loaded lipid nanocapsules significantly enhanced the decrease in IC50 rate, and the treated cells evidenced apoptosis and a premature progression of the cell cycle from G(1) to G(2)-M phase. The chemotherapeutic effect of free docetaxel on breast cancer cells is improved by its encapsulation in lipid nanocapsules. This approach has the potential to overcome some major limitations of conventional chemotherapy and may be a promising strategy for future applications in breast cancer therapy.
Keywords: lipid nanoparticles; docetaxel; drug delivery; breast cancer
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Sánchez-Moreno, P.; Boulaiz, H.; Ortega-Vinuesa, J.L.; Peula-García, J.M.; Aránega, A. Novel Drug Delivery System Based on Docetaxel-Loaded Nanocapsules as a Therapeutic Strategy Against Breast Cancer Cells. Int. J. Mol. Sci. 2012, 13, 4906-4919.
Sánchez-Moreno P, Boulaiz H, Ortega-Vinuesa JL, Peula-García JM, Aránega A. Novel Drug Delivery System Based on Docetaxel-Loaded Nanocapsules as a Therapeutic Strategy Against Breast Cancer Cells. International Journal of Molecular Sciences. 2012; 13(4):4906-4919.
Sánchez-Moreno, Paola; Boulaiz, Houria; Ortega-Vinuesa, Juan Luis; Peula-García, José Manuel; Aránega, Antonia. 2012. "Novel Drug Delivery System Based on Docetaxel-Loaded Nanocapsules as a Therapeutic Strategy Against Breast Cancer Cells." Int. J. Mol. Sci. 13, no. 4: 4906-4919.