Next Article in Journal
Genetic Diversity and Differentiation of Dendrocalamus membranaceus (Poaceae: Bambusoideae), a Declining Bamboo Species in Yunnan, China, as Based on Inter-Simple Sequence Repeat (ISSR) Analysis
Next Article in Special Issue
Biochemistry of Bacterial Multidrug Efflux Pumps
Previous Article in Journal
Density Functional Theory (DFT) Study of Triphenylamine-Based Dyes for Their Use as Sensitizers in Molecular Photovoltaics
Previous Article in Special Issue
The Intriguing Life of Autophagosomes
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2012, 13(4), 4433-4445; doi:10.3390/ijms13044433

Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes

1
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, UK
2
Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
3
Department of Musculoskeletal Biology, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, Merseyside L69 3GA, UK
*
Author to whom correspondence should be addressed.
Received: 27 February 2012 / Revised: 12 March 2012 / Accepted: 26 March 2012 / Published: 10 April 2012
(This article belongs to the Special Issue Membrane Transport)
View Full-Text   |   Download PDF [395 KB, uploaded 19 June 2014]   |  

Abstract

Ion channels play important roles in chondrocyte mechanotransduction. The transient receptor potential vanilloid (TRPV) subfamily of ion channels consists of six members. TRPV1-4 are temperature sensitive calcium-permeable, relatively non-selective cation channels whereas TRPV5 and TRPV6 show high selectivity for calcium over other cations. In this study we investigated the effect of time in culture and passage number on the expression of TRPV4, TRPV5 and TRPV6 in articular chondrocytes isolated from equine metacarpophalangeal joints. Polyclonal antibodies raised against TRPV4, TRPV5 and TRPV6 were used to compare the expression of these channels in lysates from first expansion chondrocytes (P0) and cells from passages 1–3 (P1, P2 and P3) by western blotting. TRPV4, TRPV5 and TRPV6 were expressed in all passages examined. Immunohistochemistry and immunofluorescence confirmed the presence of these channels in sections of formalin fixed articular cartilage and monolayer cultures of methanol fixed P2 chondrocytes. TRPV5 and TRPV6 were upregulated with time and passage in culture suggesting that a shift in the phenotype of the cells in monolayer culture alters the expression of these channels. In conclusion, several TRPV channels are likely to be involved in calcium signaling and homeostasis in chondrocytes. View Full-Text
Keywords: cartilage; chondrocyte; culture; passage; mechanotransduction; immunohistochemistry; immunofluorescence; dedifferentiation; TRPV4; TRPV5; TRPV6 cartilage; chondrocyte; culture; passage; mechanotransduction; immunohistochemistry; immunofluorescence; dedifferentiation; TRPV4; TRPV5; TRPV6
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hdud, I.M.; El-Shafei, A.A.; Loughna, P.; Barrett-Jolley, R.; Mobasheri, A. Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes. Int. J. Mol. Sci. 2012, 13, 4433-4445.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top