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Int. J. Mol. Sci. 2012, 13(4), 4433-4445; doi:10.3390/ijms13044433
Article

Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes

1
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Received: 27 February 2012; in revised form: 12 March 2012 / Accepted: 26 March 2012 / Published: 10 April 2012
(This article belongs to the Special Issue Membrane Transport)
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Abstract: Ion channels play important roles in chondrocyte mechanotransduction. The transient receptor potential vanilloid (TRPV) subfamily of ion channels consists of six members. TRPV1-4 are temperature sensitive calcium-permeable, relatively non-selective cation channels whereas TRPV5 and TRPV6 show high selectivity for calcium over other cations. In this study we investigated the effect of time in culture and passage number on the expression of TRPV4, TRPV5 and TRPV6 in articular chondrocytes isolated from equine metacarpophalangeal joints. Polyclonal antibodies raised against TRPV4, TRPV5 and TRPV6 were used to compare the expression of these channels in lysates from first expansion chondrocytes (P0) and cells from passages 1–3 (P1, P2 and P3) by western blotting. TRPV4, TRPV5 and TRPV6 were expressed in all passages examined. Immunohistochemistry and immunofluorescence confirmed the presence of these channels in sections of formalin fixed articular cartilage and monolayer cultures of methanol fixed P2 chondrocytes. TRPV5 and TRPV6 were upregulated with time and passage in culture suggesting that a shift in the phenotype of the cells in monolayer culture alters the expression of these channels. In conclusion, several TRPV channels are likely to be involved in calcium signaling and homeostasis in chondrocytes.
Keywords: cartilage; chondrocyte; culture; passage; mechanotransduction; immunohistochemistry; immunofluorescence; dedifferentiation; TRPV4; TRPV5; TRPV6 cartilage; chondrocyte; culture; passage; mechanotransduction; immunohistochemistry; immunofluorescence; dedifferentiation; TRPV4; TRPV5; TRPV6
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hdud, I.M.; El-Shafei, A.A.; Loughna, P.; Barrett-Jolley, R.; Mobasheri, A. Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes. Int. J. Mol. Sci. 2012, 13, 4433-4445.

AMA Style

Hdud IM, El-Shafei AA, Loughna P, Barrett-Jolley R, Mobasheri A. Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes. International Journal of Molecular Sciences. 2012; 13(4):4433-4445.

Chicago/Turabian Style

Hdud, Ismail M.; El-Shafei, Abdelrafea A.; Loughna, Paul; Barrett-Jolley, Richard; Mobasheri, Ali. 2012. "Expression of Transient Receptor Potential Vanilloid (TRPV) Channels in Different Passages of Articular Chondrocytes." Int. J. Mol. Sci. 13, no. 4: 4433-4445.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert