Int. J. Mol. Sci. 2012, 13(4), 4412-4417; doi:10.3390/ijms13044412
Article

Isolation and Characterization of Twelve Polymorphic Microsatellite Loci for the Cocoa Mirid Bug Sahlbergella Singularis

1,* email, 1email, 2email, 1email, 2email, 2email and 1email
Received: 1 March 2012; in revised form: 23 March 2012 / Accepted: 26 March 2012 / Published: 10 April 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Mirids are the primary pests affecting cocoa production in Africa, but no genetic studies have been conducted on these insects. Here we report the isolation and characterization of 12 polymorphic microsatellite loci for Sahlbergella singularis. A microsatellite-enriched genomic DNA library was developed and screened to identify marker loci. Twelve polymorphic loci were identified by screening 28 individuals collected from one presumed population in cocoa plantations in Southern Cameroon. The number of alleles ranged from 5 to 25, whereas the observed and the expected heterozygosities ranged from 0.179 to 0.786 and from 0.671 to 0.946, respectively. Tests showed significant deviations from HW equilibrium for four loci, but no linkage disequilibrium was detected at any of the loci. No cross-species amplification was observed in two other mirid pests in Africa.
Keywords: genetic markers; Miridae; Sahlbergella singularis; Distantiella theobroma; Bryocorinae
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MDPI and ACS Style

Babin, R.; Fenouillet, C.; Legavre, T.; Blondin, L.; Calatayud, C.; Risterucci, A.-M.; Chapuis, M.-P. Isolation and Characterization of Twelve Polymorphic Microsatellite Loci for the Cocoa Mirid Bug Sahlbergella Singularis. Int. J. Mol. Sci. 2012, 13, 4412-4417.

AMA Style

Babin R, Fenouillet C, Legavre T, Blondin L, Calatayud C, Risterucci A-M, Chapuis M-P. Isolation and Characterization of Twelve Polymorphic Microsatellite Loci for the Cocoa Mirid Bug Sahlbergella Singularis. International Journal of Molecular Sciences. 2012; 13(4):4412-4417.

Chicago/Turabian Style

Babin, Régis; Fenouillet, Catherine; Legavre, Thierry; Blondin, Laurence; Calatayud, Caroline; Risterucci, Ange-Marie; Chapuis, Marie-Pierre. 2012. "Isolation and Characterization of Twelve Polymorphic Microsatellite Loci for the Cocoa Mirid Bug Sahlbergella Singularis." Int. J. Mol. Sci. 13, no. 4: 4412-4417.

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