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Int. J. Mol. Sci. 2012, 13(4), 4281-4294; doi:10.3390/ijms13044281

Evolution of Microsatellite Loci of Tropical and Temperate Anguilla Eels

Department of Aquaculture, National Pingtung University of Science & Technology, Pingtung 912, Taiwan
Received: 19 January 2012 / Revised: 13 March 2012 / Accepted: 15 March 2012 / Published: 2 April 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Anguilla eels are divided into temperate and tropical eels, based on their major distributions. The present study collected two temperate eels, Anguilla japonica and Anguilla anguilla, and two tropical eels, Anguilla marmorata and Anguilla bicolor pacifica, to examine two questions: do temperate and tropical Anguilla eels have different genetic polymorphic patterns?; and do temperate Anguilla japonica and Anguilla anguilla have a closer relationship to each other than to tropical eels? In total, 274 sequences were cloned and sequenced from six conserved microsatellite loci to examine polymorphic patterns of these four catadromous eels. Different mutational events, including substitutions, and repeat-unit deletions and insertions, appeared in major regions, while different point mutations were observed in flanking regions. The results implied that parallel patterns of microsatellite sequences occurred within both tropical and temperate freshwater eels. Consensus flanking sequences of six homologous loci from each of the four species were constructed. Genetic distances ranged from 0.044 (Anguilla bicolor pacifica vs. Anguilla marmorata) to 0.061 (Anguilla marmorata vs. Anguilla anguilla). The tree topology suggests the hypothesis of Anguilla japonica and Anguilla anguilla being a sister group must be rejected.
Keywords: flanking sequence; major region; polymorphic pattern flanking sequence; major region; polymorphic pattern
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Tseng, M.-C. Evolution of Microsatellite Loci of Tropical and Temperate Anguilla Eels. Int. J. Mol. Sci. 2012, 13, 4281-4294.

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