Int. J. Mol. Sci. 2012, 13(4), 4089-4100; doi:10.3390/ijms13044089
Response Surface Methodology to Optimize Enzymatic Preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi
1
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
2
The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China
3
China-Japan Union Hospital, Jilin University, Changchun 130033, China
†
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 3 February 2012 / Revised: 8 March 2012 / Accepted: 19 March 2012 / Published: 28 March 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
Abstract
In the present work, we reported the enzymatic preparation of deapio-platycodin D (dPD) and platycodin D (PD) optimized by response surface methodology (RSM) from Radix Platycodi. During investigation of the hydrolysis of crude platycosides by various glycoside hydrolases, snailase showed a strong ability to transform deapio-platycoside E (dPE) and platycoside E (PE) into dPD and PD with 100% conversion. RSM was used to optimize the effects of the reaction temperature (35–45 °C), enzyme load (5–20%), and reaction time (4–24 h) on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of dPD and PD conversion yield. The optimum preparation conditions were as follows: temperature, 43 °C; enzyme load, 15%; reaction time, 22 h. The biotransformation pathways were dPE→dPD3→dPD and PE→PD3→PD, respectively. The determined method may be highly applicable for the enzymatic preparation of dPD and PD for medicinal purposes and also for commercial use.Keywords:
snailase; deapio-platycodin D; platycodin D; radix platycodi; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Li, W.; Zhao, L.-C.; Wang, Z.; Zheng, Y.-N.; Liang, J.; Wang, H. Response Surface Methodology to Optimize Enzymatic Preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi. Int. J. Mol. Sci. 2012, 13, 4089-4100.
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