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Int. J. Mol. Sci. 2012, 13(4), 4003-4008; doi:10.3390/ijms13044003
Short Note

Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)

1,†
, 1,2,†
, 3
, 1
 and 4,*
Received: 6 February 2012; in revised form: 15 March 2012 / Accepted: 19 March 2012 / Published: 23 March 2012
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Abstract: The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.
Keywords: Chimaphila japonica; microsatellite marker; polymorphism; population genetics Chimaphila japonica; microsatellite marker; polymorphism; population genetics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liu, Z.; Zhao, Q.; Zhou, J.; Peng, H.; Yang, J. Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae). Int. J. Mol. Sci. 2012, 13, 4003-4008.

AMA Style

Liu Z, Zhao Q, Zhou J, Peng H, Yang J. Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae). International Journal of Molecular Sciences. 2012; 13(4):4003-4008.

Chicago/Turabian Style

Liu, Zhenwen; Zhao, Qianru; Zhou, Jing; Peng, Hua; Yang, Junbo. 2012. "Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)." Int. J. Mol. Sci. 13, no. 4: 4003-4008.


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