Next Article in Journal
Pharmacokinetic Comparison of Ferulic Acid in Normal and Blood Deficiency Rats after Oral Administration of Angelica sinensis, Ligusticum chuanxiong and Their Combination
Previous Article in Journal
A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli
Int. J. Mol. Sci. 2012, 13(3), 3563-3582; doi:10.3390/ijms13033563
Article

Autophagy in Premature Senescent Cells Is Activated via AMPK Pathway

1,†
, 2,†
 and 1,*
Received: 7 February 2012; in revised form: 23 February 2012 / Accepted: 6 March 2012 / Published: 16 March 2012
View Full-Text   |   Download PDF [777 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Autophagy is a highly regulated intracellular process involved in the turnover of most cellular constituents and in the maintenance of cellular homeostasis. In this study, we show that the activity of autophagy increases in H2O2 or RasV12-induced senescent fibroblasts. Inhibiting autophagy promotes cell apoptosis in senescent cells, suggesting that autophagy activation plays a cytoprotective role. Furthermore, our data indicate that the increase of autophagy in senescent cells is linked to the activation of transcription factor FoxO3A, which blocks ATP generation by transcriptionally up-regulating the expression of PDK4, an inhibitor of pyruvate dehydrogenase complex, thus leading to AMPK activation and mTOR inhibition. These findings suggest a novel mechanism by which FoxO3A factors can activate autophagy via metabolic alteration.
Keywords: autophagy; senescent cells; apoptosis; FoxO3A; AMPK; fibroblasts autophagy; senescent cells; apoptosis; FoxO3A; AMPK; fibroblasts
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Guo, L.; Xie, B.; Mao, Z. Autophagy in Premature Senescent Cells Is Activated via AMPK Pathway. Int. J. Mol. Sci. 2012, 13, 3563-3582.

AMA Style

Guo L, Xie B, Mao Z. Autophagy in Premature Senescent Cells Is Activated via AMPK Pathway. International Journal of Molecular Sciences. 2012; 13(3):3563-3582.

Chicago/Turabian Style

Guo, Liujing; Xie, Bushan; Mao, Zebin. 2012. "Autophagy in Premature Senescent Cells Is Activated via AMPK Pathway." Int. J. Mol. Sci. 13, no. 3: 3563-3582.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert