Next Article in Journal
The Hypoglycemic Effect of the Kelp on Diabetes Mellitus Model Induced by Alloxan in Rats
Previous Article in Journal
Valuable Nutrients and Functional Bioactives in Different Parts of Olive (Olea europaea L.)—A Review
Int. J. Mol. Sci. 2012, 13(3), 3341-3353; doi:10.3390/ijms13033341

Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine

1,†,* , 2,†
1 State Key Laboratory of Chem/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, China 2 Hunan Provincial Tumor Hospital, Changsha 410013, China 3 Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China 4 Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 19 January 2012 / Revised: 1 February 2012 / Accepted: 1 March 2012 / Published: 12 March 2012
View Full-Text   |   Download PDF [481 KB, uploaded 19 June 2014]   |   Browse Figures


Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential enrichment (SELEX). Recent advancements in SELEX technology have extended aptamer selection from comparatively simple mixtures of purified proteins to whole living cells, and now cell-based SELEX (or cell-SELEX) can isolate aptamers that bind to specific target cells. Combined with nanotechnology, microchips, microfluidic devices, RNAi and other advanced technologies, cell-SELEX represents an integrated platform providing ultrasensitive and highly specific tools for clinical medicine. In this review, we describe the recent progress made in the application of cell-SELEX for diagnosis, therapy and biomarker discovery.
Keywords: aptamer; SELEX; molecular medicine aptamer; SELEX; molecular medicine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Ye, M.; Hu, J.; Peng, M.; Liu, J.; Liu, J.; Liu, H.; Zhao, X.; Tan, W. Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine. Int. J. Mol. Sci. 2012, 13, 3341-3353.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert