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• Ye, M
• Hu, J
• Peng, M
• Liu, J
• Liu, J
• Liu, H
• Zhao, X
• Tan, W
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• Hu, J
• Peng, M
• Liu, J
• Liu, J
• Liu, H
• Zhao, X
• Tan, W
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• Hu, J
• Peng, M
• Liu, J
• Liu, J
• Liu, H
• Zhao, X
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Int. J. Mol. Sci. 2012, 13(3), 3341-3353; doi:10.3390/ijms13033341

Review

Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine

Mao Ye ^1,†,* , Jun Hu ^2,† , Minyuan Peng ^3,† , Jing Liu ^3,† , Jun Liu ³ , Huixia Liu ³ , Xielan Zhao ³  and Weihong Tan ^1,4,*

¹ State Key Laboratory of Chem/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, China ² Hunan Provincial Tumor Hospital, Changsha 410013, China ³ Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China ⁴ Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA ^† These authors contributed equally to this work.

* Authors to whom correspondence should be addressed.

Received: 19 January 2012; in revised form: 1 February 2012 / Accepted: 1 March 2012 / Published: 12 March 2012

Download PDF Full-Text [481 KB, uploaded 12 March 2012 09:15 CET]

Abstract: Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential enrichment (SELEX). Recent advancements in SELEX technology have extended aptamer selection from comparatively simple mixtures of purified proteins to whole living cells, and now cell-based SELEX (or cell-SELEX) can isolate aptamers that bind to specific target cells. Combined with nanotechnology, microchips, microfluidic devices, RNAi and other advanced technologies, cell-SELEX represents an integrated platform providing ultrasensitive and highly specific tools for clinical medicine. In this review, we describe the recent progress made in the application of cell-SELEX for diagnosis, therapy and biomarker discovery.

Keywords: aptamer; SELEX; molecular medicine

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