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Int. J. Mol. Sci. 2012, 13(3), 3341-3353; doi:10.3390/ijms13033341

Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine

1,†,* , 2,†
,
3,†
,
3,†
,
3
,
3
,
3
and
1,4,*
1
State Key Laboratory of Chem/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, China
2
Hunan Provincial Tumor Hospital, Changsha 410013, China
3
Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China
4
Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 19 January 2012 / Revised: 1 February 2012 / Accepted: 1 March 2012 / Published: 12 March 2012
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Abstract

Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential enrichment (SELEX). Recent advancements in SELEX technology have extended aptamer selection from comparatively simple mixtures of purified proteins to whole living cells, and now cell-based SELEX (or cell-SELEX) can isolate aptamers that bind to specific target cells. Combined with nanotechnology, microchips, microfluidic devices, RNAi and other advanced technologies, cell-SELEX represents an integrated platform providing ultrasensitive and highly specific tools for clinical medicine. In this review, we describe the recent progress made in the application of cell-SELEX for diagnosis, therapy and biomarker discovery. View Full-Text
Keywords: aptamer; SELEX; molecular medicine aptamer; SELEX; molecular medicine
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ye, M.; Hu, J.; Peng, M.; Liu, J.; Liu, J.; Liu, H.; Zhao, X.; Tan, W. Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine. Int. J. Mol. Sci. 2012, 13, 3341-3353.

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