Next Article in Journal
Prediction of Genomic Islands in Three Bacterial Pathogens of Pneumonia
Previous Article in Journal
Comparative Evolution of S7 Intron 1 and Ribosomal Internal Transcribed Spacer in Coilia nasus (Clupeiformes: Engraulidae)
Previous Article in Special Issue
Major Phenolic Compounds, Antioxidant Capacity and Antidiabetic Potential of Rice Bean (Vigna umbellata L.) in China
Article Menu

Export Article

Open AccessReview
Int. J. Mol. Sci. 2012, 13(3), 3101-3133; doi:10.3390/ijms13033101

Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives

1
Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa
2
Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa
*
Author to whom correspondence should be addressed.
Received: 7 December 2011 / Revised: 8 February 2012 / Accepted: 1 March 2012 / Published: 7 March 2012
(This article belongs to the Special Issue Advances in Nutraceutical Research)
View Full-Text   |   Download PDF [460 KB, 19 June 2014; original version 19 June 2014]   |  

Abstract

Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays.
Keywords: antioxidant; bioactive phytochemicals; biochemical detection; enzyme inhibition; HPLC; receptor affinity antioxidant; bioactive phytochemicals; biochemical detection; enzyme inhibition; HPLC; receptor affinity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Malherbe, C.J.; de Beer, D.; Joubert, E. Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives. Int. J. Mol. Sci. 2012, 13, 3101-3133.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top