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Int. J. Mol. Sci. 2012, 13(3), 3101-3133; doi:10.3390/ijms13033101
Review

Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives

1,* , 1
 and
1,2
1 Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa 2 Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa
* Author to whom correspondence should be addressed.
Received: 7 December 2011 / Revised: 8 February 2012 / Accepted: 1 March 2012 / Published: 7 March 2012
(This article belongs to the Special Issue Advances in Nutraceutical Research)
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Abstract

Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays.
Keywords: antioxidant; bioactive phytochemicals; biochemical detection; enzyme inhibition; HPLC; receptor affinity antioxidant; bioactive phytochemicals; biochemical detection; enzyme inhibition; HPLC; receptor affinity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Malherbe, C.J.; de Beer, D.; Joubert, E. Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives. Int. J. Mol. Sci. 2012, 13, 3101-3133.

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