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Int. J. Mol. Sci. 2012, 13(3), 3101-3133; doi:10.3390/ijms13033101
Review

Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives

1,* , 1
 and
1,2
Received: 7 December 2011 / Revised: 8 February 2012 / Accepted: 1 March 2012 / Published: 7 March 2012
(This article belongs to the Special Issue Advances in Nutraceutical Research)
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Abstract

Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays.
Keywords: antioxidant; bioactive phytochemicals; biochemical detection; enzyme inhibition; HPLC; receptor affinity antioxidant; bioactive phytochemicals; biochemical detection; enzyme inhibition; HPLC; receptor affinity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Malherbe, C.J.; de Beer, D.; Joubert, E. Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives. Int. J. Mol. Sci. 2012, 13, 3101-3133.

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