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Int. J. Mol. Sci. 2012, 13(12), 17275-17289; doi:10.3390/ijms131217275
Article

Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide

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Received: 21 August 2012; in revised form: 17 November 2012 / Accepted: 4 December 2012 / Published: 17 December 2012
(This article belongs to the Section Green Chemistry)
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Abstract: In this study, an experiment was designed to optimize the synthesis of seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na2SeO3 to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na2SeO3 to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner.
Keywords: seleno-Capparis spionosa L. polysaccharide(Se-CSPS); reaction optimization; response surface methodology; characterization; cytotoxic activity seleno-Capparis spionosa L. polysaccharide(Se-CSPS); reaction optimization; response surface methodology; characterization; cytotoxic activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ji, Y.-B.; Dong, F.; Lang, L.; Zhang, L.-W.; Miao, J.; Liu, Z.-F.; Jin, L.-N.; Hao, Y. Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide. Int. J. Mol. Sci. 2012, 13, 17275-17289.

AMA Style

Ji Y-B, Dong F, Lang L, Zhang L-W, Miao J, Liu Z-F, Jin L-N, Hao Y. Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide. International Journal of Molecular Sciences. 2012; 13(12):17275-17289.

Chicago/Turabian Style

Ji, Yu-Bin; Dong, Fang; Lang, Lang; Zhang, Ling-Wen; Miao, Jing; Liu, Zhen-Feng; Jin, Li-Na; Hao, Ying. 2012. "Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide." Int. J. Mol. Sci. 13, no. 12: 17275-17289.



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