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Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide
Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China
Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China
* Author to whom correspondence should be addressed.
Received: 21 August 2012; in revised form: 17 November 2012 / Accepted: 4 December 2012 / Published: 17 December 2012
Abstract: In this study, an experiment was designed to optimize the synthesis of seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na2SeO3 to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na2SeO3 to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner.
Keywords: seleno-Capparis spionosa L. polysaccharide(Se-CSPS); reaction optimization; response surface methodology; characterization; cytotoxic activity
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Ji, Y.-B.; Dong, F.; Lang, L.; Zhang, L.-W.; Miao, J.; Liu, Z.-F.; Jin, L.-N.; Hao, Y. Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide. Int. J. Mol. Sci. 2012, 13, 17275-17289.
Ji Y-B, Dong F, Lang L, Zhang L-W, Miao J, Liu Z-F, Jin L-N, Hao Y. Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide. International Journal of Molecular Sciences. 2012; 13(12):17275-17289.
Ji, Yu-Bin; Dong, Fang; Lang, Lang; Zhang, Ling-Wen; Miao, Jing; Liu, Zhen-Feng; Jin, Li-Na; Hao, Ying. 2012. "Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide." Int. J. Mol. Sci. 13, no. 12: 17275-17289.