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Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells
Chair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 24 October 2012; in revised form: 13 November 2012 / Accepted: 14 November 2012 / Published: 20 November 2012
Abstract: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in cancer cells without toxicity to normal cells. TRAIL binds to death receptors, TRAIL-R1 (DR4) and TRAIL-R2 (DR5) expressed on cancer cell surface and activates apoptotic pathways. Endogenous TRAIL plays an important role in immune surveillance and defense against cancer cells. However, as more tumor cells are reported to be resistant to TRAIL mediated death, it is important to search for and develop new strategies to overcome this resistance. Chalcones can sensitize cancer cells to TRAIL-induced apoptosis. We examined the cytotoxic and apoptotic effects of TRAIL in combination with four chalcones: chalcone, isobavachalcone, licochalcone A and xanthohumol on HeLa cancer cells. The cytotoxicity was measured by MTT and LDH assays. The apoptosis was detected using annexin V-FITC staining by flow cytometry and fluorescence microscopy. Death receptor expression was analyzed using flow cytometry. The decreased expression of death receptors in cancer cells may be the cause of TRAIL-resistance. Chalcones enhance TRAIL-induced apoptosis in HeLa cells through increased expression of TRAIL-R2. Our study has indicated that chalcones augment the antitumor activity of TRAIL and confirm their cancer chemopreventive properties.
Keywords: TRAIL; chalcones; apoptosis; death receptors; cancer cells; chemoprevention
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Szliszka, E.; Jaworska, D.; Ksek, M.; Czuba, Z.P.; Król, W. Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells. Int. J. Mol. Sci. 2012, 13, 15343-15359.
Szliszka E, Jaworska D, Ksek M, Czuba ZP, Król W. Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells. International Journal of Molecular Sciences. 2012; 13(11):15343-15359.
Szliszka, Ewelina; Jaworska, Dagmara; Ksek, Małgorzata; Czuba, Zenon P.; Król, Wojciech. 2012. "Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells." Int. J. Mol. Sci. 13, no. 11: 15343-15359.