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Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models
Institute for NanoBiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China
College of Life Science, Jianghan University, Wuhan 430056, Hubei, China
National Engineering Research Center for solid state fermentation, Luzhou 640040, Sichuan, China
* Author to whom correspondence should be addressed.
Received: 13 July 2012; in revised form: 8 November 2012 / Accepted: 12 November 2012 / Published: 19 November 2012
Abstract: Ionic self-assembly of the peptide RADARADARADARADA (RADA16-1) may form a well-defined nanofiber and eventually a hydrogel scaffold, with a water content of over 99.5%. This leads to the establishment of a nanofiber barrier that can be used to achieve complete hemostasis in less than 20 s in multiple tissues and in a variety of different wounds. In the present study, the nanofiber scaffolds of RADA16-1 peptide were sonicated into smaller fragments to identify possible molecular mechanisms underlying the rapid cessation of bleeding associated with these materials. Atomic force microscopy (AFM), circular dichroism (CD), and rheometry were also used to evaluate the re-assembly kinetics of this peptide. A bleeding control experiment was performed in animal models to uncover the molecular mechanisms underlying this fast hemostasis. In this way, these sonicated fragments not only quickly reassembled into nanofibers indistinguishable from the original material, but the degree of reassembly was also correlated with an increase in the rigidity of the scaffold and increased as the time required for hemostasis increased.
Keywords: RADA16-1; nanofiber scaffolds; self-assembly; hemostasis
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Wang, T.; Zhong, X.; Wang, S.; Lv, F.; Zhao, X. Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models. Int. J. Mol. Sci. 2012, 13, 15279-15290.
Wang T, Zhong X, Wang S, Lv F, Zhao X. Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models. International Journal of Molecular Sciences. 2012; 13(11):15279-15290.
Wang, Ting; Zhong, Xiaozhong; Wang, Songtao; Lv, Fei; Zhao, Xiaojun. 2012. "Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models." Int. J. Mol. Sci. 13, no. 11: 15279-15290.