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Healing, Antioxidant and Cytoprotective Properties of Indigofera truxillensis in Different Models of Gastric Ulcer in Rats
Department of Biological Sciences, Federal University of Goiás, 75704-020, Catalão, Goiás, Brazil
Department of Structural and Functional Biology, Biology Institute, Campinas University, 13083-865, Campinas, São Paulo, Brazil
Department of Pharmacology, Faculty of Medical Sciences, University of Campinas, 13083-887, Campinas, São Paulo, Brazil
Department of Organic Chemistry, Institute of Chemistry, São Paulo State University, 14800-900, Araraquara, São Paulo, Brazil
Department of Physiology, Institute of Biosciences, São Paulo State University, 18618-000, Botucatu, São Paulo, Brazil
* Author to whom correspondence should be addressed.
Received: 28 June 2012; in revised form: 27 August 2012 / Accepted: 8 October 2012 / Published: 15 November 2012
Abstract: The present study evaluated the antiulcerogenic activity and mechanisms of the aqueous (AqF 100 mg/kg) and ethyl acetate (AcF 50 mg/kg) fractions from Indigofera truxillensis leaves. This dose was selected to assess its activity on ulcer healing and its action on gastric acid and mucus secretion, prostaglandin production and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rd)). Gastric ulcer was induced by absolute ethanol. Antisecretory action, mucus and prostaglandin production, healing and antioxidant enzyme activities were evaluated for both fractions. AqF and AcF significantly inhibited the gastric mucosal damage caused by ethanol. This effect was statistically significant at 100 and 50 mg/kg compared with the vehicle. Neither fraction interfered with gastric secretion. AcF increased the PGE2 production, and both fractions increased mucus production. l-NAME did not alter the gastroprotection exerted by the fractions, but N-ethylmaleimide attenuated only AcF. In the ischemia/reperfusion model both fractions inhibited the mucosal damage. AcF increased SOD, GSH-Px and GSH-Rd activity, but AqF increased only SOD and GSH-Px. In the acetic acid-induced ulcer model AcF only accelerated ulcer healing. These results showed that Indigofera truxillensis acted as a gastroprotective agent, stimulating protective factors and antioxidants enzymes.
Keywords: Indigofera truxillensis; gastric ulcer; medicinal plants; antioxidant enzymes
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Luiz-Ferreira, A.; Cola, M.; Barbastefano, V.; de-Faria, F.M.; Almeida, A.B.A.; Farias-Silva, E.; Calvo, T.R.; Hiruma-Lima, C.A.; Vilegas, W.; Souza-Brito, A.R.M. Healing, Antioxidant and Cytoprotective Properties of Indigofera truxillensis in Different Models of Gastric Ulcer in Rats. Int. J. Mol. Sci. 2012, 13, 14973-14991.
Luiz-Ferreira A, Cola M, Barbastefano V, de-Faria FM, Almeida ABA, Farias-Silva E, Calvo TR, Hiruma-Lima CA, Vilegas W, Souza-Brito ARM. Healing, Antioxidant and Cytoprotective Properties of Indigofera truxillensis in Different Models of Gastric Ulcer in Rats. International Journal of Molecular Sciences. 2012; 13(11):14973-14991.
Luiz-Ferreira, Anderson; Cola, Maira; Barbastefano, Victor; de-Faria, Felipe M.; Almeida, Ana B.A.; Farias-Silva, Elisângela; Calvo, Tamara R.; Hiruma-Lima, Clélia A.; Vilegas, Wagner; Souza-Brito, Alba R.M. 2012. "Healing, Antioxidant and Cytoprotective Properties of Indigofera truxillensis in Different Models of Gastric Ulcer in Rats." Int. J. Mol. Sci. 13, no. 11: 14973-14991.