Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2012, 13(11), 13894-13910; doi:10.3390/ijms131113894
Article

Profiling the Proteome of Exhaled Breath Condensate in Healthy Smokers and COPD Patients by LC-MS/MS

1
, 2
, 3
, 4
, 4
, 3
, 5
, 4
, 3
, 6
, 7
 and 1,*
Received: 29 August 2012; in revised form: 16 October 2012 / Accepted: 18 October 2012 / Published: 29 October 2012
(This article belongs to the collection Advances in Proteomic Research)
View Full-Text   |   Download PDF [1251 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Three pools of exhaled breath condensate (EBC) from non-smokers plus healthy smokers (NS + HS, n = 45); chronic obstructive pulmonary disease (COPD) without emphysema (COPD, n = 15) and subjects with pulmonary emphysema associated with α1-antitrypsin deficiency (AATD, n = 23) were used for an exploratory proteomic study aimed at generating fingerprints of these groups that can be used in future pathophysiological and perhaps even clinical research. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was the platform applied for this hypothesis-free investigation. Analysis of pooled specimens resulted in the production of a “fingerprint” made of 44 proteins for NS/HS; 17 for COPD and 15 for the group of AATD subjects. Several inflammatory cytokines (IL-1α, IL-1β, IL-2; IL-12, α and β subunits, IL-15, interferon α and γ, tumor necrosis factor α); Type I and II cytokeratins; two SP-A isoforms; Calgranulin A and B and α1-antitrypsin were detected and validated through the use of surface enhanced laser-desorption ionization mass spectrometry (SELDI-MS) and/or by Western blot (WB) analysis. These results are the prelude of quantitative studies aimed at identifying which of these proteins hold promise as identifiers of differences that could distinguish healthy subjects from patients.
Keywords: EBC; COPD; proteomics; LC-MS/MS EBC; COPD; proteomics; LC-MS/MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Fumagalli, M.; Ferrari, F.; Luisetti, M.; Stolk, J.; Hiemstra, P.S.; Capuano, D.; Viglio, S.; Fregonese, L.; Cerveri, I.; Corana, F.; Tinelli, C.; Iadarola, P. Profiling the Proteome of Exhaled Breath Condensate in Healthy Smokers and COPD Patients by LC-MS/MS. Int. J. Mol. Sci. 2012, 13, 13894-13910.

AMA Style

Fumagalli M, Ferrari F, Luisetti M, Stolk J, Hiemstra PS, Capuano D, Viglio S, Fregonese L, Cerveri I, Corana F, Tinelli C, Iadarola P. Profiling the Proteome of Exhaled Breath Condensate in Healthy Smokers and COPD Patients by LC-MS/MS. International Journal of Molecular Sciences. 2012; 13(11):13894-13910.

Chicago/Turabian Style

Fumagalli, Marco; Ferrari, Fabio; Luisetti, Maurizio; Stolk, Jan; Hiemstra, Pieter S.; Capuano, Daniela; Viglio, Simona; Fregonese, Laura; Cerveri, Isa; Corana, Federica; Tinelli, Carmine; Iadarola, Paolo. 2012. "Profiling the Proteome of Exhaled Breath Condensate in Healthy Smokers and COPD Patients by LC-MS/MS." Int. J. Mol. Sci. 13, no. 11: 13894-13910.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert