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Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women
Department of Morphology, (Campus II), Federal University of Goiás, 74001-970, Goiânia, GO, Brazil
College of Health Sciences, Methodist University of Piracicaba, Rodovia Açucar, Km 151, 13400-911, Piracicaba, SP, Brazil
Adventist University Center São Paulo, 13165-970, Engenheiro Coellho, SP, Brazil
Federal University of São Carlos, Rodovia Washington Luís, km 235-SP-310, 13565-905, Sao Carlos, SP, Brazil
University of São Paulo, Lab Applied Molecular Biology and Pharmacogenomics, Federal University of São Paulo, Av. Prof. Lineu Prestes, 58005508-900, Sao Paulo, SP, Brazil
* Author to whom correspondence should be addressed.
Received: 27 August 2012; in revised form: 25 September 2012 / Accepted: 10 October 2012 / Published: 22 October 2012
Abstract: This study examined the association of estrogen receptor alpha gene (ESR1) polymorphisms with cardiorespiratory and metabolic parameters in young women. In total, 354 healthy women were selected for cardiopulmonary exercise testing and short-term heart rate (HR) variability (HRV) evaluation. The HRV analysis was determined by the temporal indices rMSSD (square root of the mean squared differences of successive R–R intervals (RRi) divided by the number of RRi minus one), SDNN (root mean square of differences from mean RRi, divided by the number of RRi) and power spectrum components by low frequency (LF), high frequency (HF) and LF/HF ratio. Blood samples were obtained for serum lipids, estradiol and DNA extraction. ESR1 rs2234693 and rs9340799 polymorphisms were analyzed by PCR and fragment restriction analysis. HR and oxygen uptake (VO2) values did not differ between the ESR1 polymorphisms with respect to autonomic modulation. We not find a relationship between ESR1 T–A, T–G, C–A and C–G haplotypes and cardiorespiratory and metabolic variables. Multiple linear regression analysis demonstrated that VO2, total cholesterol and triglycerides influence HRV (p < 0.05). The results suggest that ESR1 variants have no effect on cardiorespiratory and metabolic variables, while HRV indices are influenced by aerobic capacity and lipids in healthy women.
Keywords: estrogen receptor-α gene polymorphisms; heart rate variability; aerobic capacity; lipids
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Rebelo, A.C.; Verlengia, R.; Kunz, V.; Tamburus, N.; Cerda, A.; Hirata, R.; Hirata, M.; Silva, E. Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women. Int. J. Mol. Sci. 2012, 13, 13691-13703.
Rebelo AC, Verlengia R, Kunz V, Tamburus N, Cerda A, Hirata R, Hirata M, Silva E. Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women. International Journal of Molecular Sciences. 2012; 13(10):13691-13703.
Rebelo, Ana Cristina; Verlengia, Rozangela; Kunz, Vandeni; Tamburus, Nayara; Cerda, Alvaro; Hirata, Rosario; Hirata, Mario; Silva, Ester. 2012. "Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women." Int. J. Mol. Sci. 13, no. 10: 13691-13703.