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Detection of Ricin Intoxication in Mice Using Serum Peptide Profiling by MALDI-TOF/MS
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun, Jilin 130122, China
Zoonosis Prevention and Control Key Laboratory, 666 West Liuying Road, Changchun, Jilin 130122, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 4 September 2012; in revised form: 20 September 2012 / Accepted: 11 October 2012 / Published: 22 October 2012
Abstract: Ricin toxin has been regarded as one of the most potent poisons in the plant kingdom, and there is no effective therapeutic countermeasure or licensed vaccine against it. Consequently, early detection of ricin intoxication is necessary. In this study, we took mice as test subjects, and used the technique of Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) and ClinProt™ microparticle beads to set up an effective detection model with an accuracy of almost 100%. Eighty-two peaks in the mass range 1000–10,000 m/z were detected by ClinProTools software, and five different peaks with m/z of 4982.49, 1333.25, 1537.86, 4285.05 and 2738.88 had the greatest contribution to the accuracy and sensitivity of this model. They may therefore provide biomarkers for ricin intoxication.
Keywords: ricin; MALDI-TOF-MS; detection model
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Zhao, S.; Liu, W.-S.; Wang, M.; Li, J.; Sun, Y.; Li, N.; Hou, F.; Wan, J.-Y.; Li, Z.; Qian, J.; Liu, L. Detection of Ricin Intoxication in Mice Using Serum Peptide Profiling by MALDI-TOF/MS. Int. J. Mol. Sci. 2012, 13, 13704-13712.
Zhao S, Liu W-S, Wang M, Li J, Sun Y, Li N, Hou F, Wan J-Y, Li Z, Qian J, Liu L. Detection of Ricin Intoxication in Mice Using Serum Peptide Profiling by MALDI-TOF/MS. International Journal of Molecular Sciences. 2012; 13(10):13704-13712.
Zhao, Siyan; Liu, Wen-Sen; Wang, Meng; Li, Jiping; Sun, Yucheng; Li, Nan; Hou, Feng; Wan, Jia-Yu; Li, Zhongyi; Qian, Jun; Liu, Linna. 2012. "Detection of Ricin Intoxication in Mice Using Serum Peptide Profiling by MALDI-TOF/MS." Int. J. Mol. Sci. 13, no. 10: 13704-13712.