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Icariin Ameliorates Streptozotocin-Induced Diabetic Retinopathy in Vitro and in Vivo
Department of Ophthalmology, Beijing ChaoYang Hospital, Capital Medical University, Beijing 100043, China
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 8 November 2011; in revised form: 22 December 2011 / Accepted: 28 December 2011 / Published: 16 January 2012
Abstract: This study investigated the effect of Icariin (ICA) supplementation on diabetic retinopathy (DR) in a streptozotocin-induced diabetic rat model system. Fifty Sprague Dawley rats were randomly distributed into a control group and a streptozotocin-induced diabetes group. Diabetic rats were randomly divided into two groups; one group received ICA 5 mg/kg/day for 12 weeks by oral gavage; the other group received saline gavage as a placebo. Retinal morphological changes, endothelial markers (RECA), collagen IV (Col-IV), vascular endothelial growth factor (VEGF), and neuropathic changes (Thy-1 and Brn3a expression) of the retinal ganglion cells (RGCs) were investigated. The effects of ICA at various concentrations (0, 101, 102, 103 nmol/mL) on neurite growth were investigated also in retinal ganglion cells (RGC) cultured from both diabetic and normal animals. Numerous pathological changes (deceased expression of RECA, VEGF, Thy-1, and Brn3a as well as decreased Collagen IV and Müller cell content) were noted in the retinal vessels of diabetic rats; these changes were attenuated in diabetic animals that received ICA. ICA enhanced neurite growth in RGC from both normal rats and diabetic rats in a dose dependent fashion. ICA may be useful in the treatment of diabetic retinopathy. Further investigations are indicated.
Keywords: icariin; streptozotocin; diabetes; retina; diabetic retinopathy
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Xin, H.; Zhou, F.; Liu, T.; Li, G.-Y.; Liu, J.; Gao, Z.-Z.; Bai, G.-Y.; Lu, H.; Xin, Z.-C. Icariin Ameliorates Streptozotocin-Induced Diabetic Retinopathy in Vitro and in Vivo. Int. J. Mol. Sci. 2012, 13, 866-878.
Xin H, Zhou F, Liu T, Li G-Y, Liu J, Gao Z-Z, Bai G-Y, Lu H, Xin Z-C. Icariin Ameliorates Streptozotocin-Induced Diabetic Retinopathy in Vitro and in Vivo. International Journal of Molecular Sciences. 2012; 13(1):866-878.
Xin, Hua; Zhou, Feng; Liu, Tao; Li, Guang-Yong; Liu, Jing; Gao, Zhe-Zhu; Bai, Guang-Yi; Lu, Hong; Xin, Zhong-Cheng. 2012. "Icariin Ameliorates Streptozotocin-Induced Diabetic Retinopathy in Vitro and in Vivo." Int. J. Mol. Sci. 13, no. 1: 866-878.