Int. J. Mol. Sci. 2012, 13(1), 552-557; doi:10.3390/ijms13010552
Development of Novel Microsatellite Markers in the Omei Treefrog (Rhacophorus omeimontis)
Animal Behaviour Research Group, College of Life Sciences, Central China Normal University, 152 Luoyulu, Hongshan District, Wuhan 430079, China
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Received: 13 December 2011 / Revised: 22 December 2011 / Accepted: 28 December 2011 / Published: 5 January 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract
Eleven novel microsatellite markers were developed and characterized for the Omei treefrog (Rhacophorus omeimontis) using the fast isolation by AFLP of sequences containing repeats method. Polymorphism of each locus was tested in 24 individuals from two wild populations. The number of alleles per locus ranged from 4 to 15, the average observed and expected heterozygosity per locus ranged from 0.250 to 0.839 and from 0.562 to 0.914, respectively. Two of the 11 microsatellite loci showed significant deviations from Hardy-Weinberg equilibrium. Two locus pairs showed significant linkage disequilibrium. Neither evidence of scoring error due to stuttering nor evidence of large allele dropout was found at all of the 11 loci, but evidence of null alleles was indicated at two loci because of general excess of homozygotes for most allele size classes. These polymorphic loci will be useful markers in studying mate choice of the Omei treefrog.Keywords:
Omei treefrog (Rhacophorus omeimontis); microsatellite markers; heterozygosity; mate choice
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Zhao, M.; Zhang, R.; Li, C.; Mu, T.; Wei, S.; Li, X.; Wu, H. Development of Novel Microsatellite Markers in the Omei Treefrog (Rhacophorus omeimontis). Int. J. Mol. Sci. 2012, 13, 552-557.
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