Int. J. Mol. Sci. 2012, 13(1), 1154-1160; doi:10.3390/ijms13011154
Article

Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)

Yanjiao Yuan 1,2 email, Shufang Liu 2,* email, Cuicui Bai 2,3 email, Hongbo Liu 2,3 email and Zhimeng Zhuang 2 email
1 Ocean University of Shanghai, Shanghai 201306, China 2 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China 3 Ocean University of Dalian, Dalian 116023, China
* Author to whom correspondence should be addressed.
Received: 23 December 2011; in revised form: 12 January 2012 / Accepted: 13 January 2012 / Published: 20 January 2012
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Abstract: Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)13—enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The observed and expected heterozygosities ranged from 0.063 to 0.896 and from 0.137 to 0.953, with averages of 0.519 and 0.633, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers would be useful for analyzing the population genetic structure to make conservation and management decisions for S. esculenta.
Keywords: golden cuttlefish; Sepia esculenta; microsatellite; population structure

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MDPI and ACS Style

Yuan, Y.; Liu, S.; Bai, C.; Liu, H.; Zhuang, Z. Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta). Int. J. Mol. Sci. 2012, 13, 1154-1160.

AMA Style

Yuan Y, Liu S, Bai C, Liu H, Zhuang Z. Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta). International Journal of Molecular Sciences. 2012; 13(1):1154-1160.

Chicago/Turabian Style

Yuan, Yanjiao; Liu, Shufang; Bai, Cuicui; Liu, Hongbo; Zhuang, Zhimeng. 2012. "Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)." Int. J. Mol. Sci. 13, no. 1: 1154-1160.

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