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Int. J. Mol. Sci. 2012, 13(1), 1154-1160; doi:10.3390/ijms13011154

Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)

1
Ocean University of Shanghai, Shanghai 201306, China
2
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
3
Ocean University of Dalian, Dalian 116023, China
*
Author to whom correspondence should be addressed.
Received: 23 December 2011 / Revised: 12 January 2012 / Accepted: 13 January 2012 / Published: 20 January 2012
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Abstract

Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)13—enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The observed and expected heterozygosities ranged from 0.063 to 0.896 and from 0.137 to 0.953, with averages of 0.519 and 0.633, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers would be useful for analyzing the population genetic structure to make conservation and management decisions for S. esculenta. View Full-Text
Keywords: golden cuttlefish; Sepia esculenta; microsatellite; population structure golden cuttlefish; Sepia esculenta; microsatellite; population structure
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yuan, Y.; Liu, S.; Bai, C.; Liu, H.; Zhuang, Z. Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta). Int. J. Mol. Sci. 2012, 13, 1154-1160.

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