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Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr)
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
* Author to whom correspondence should be addressed.
Received: 6 December 2011; in revised form: 27 December 2011 / Accepted: 11 January 2012 / Published: 19 January 2012
Abstract: PISTILLATA (PI)-like genes are crucial regulators of flowering in angiosperms. A homologue of PI, designated as AcPI (Genbank accession number HQ717796), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcPI is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids. The molecular weight was 2.29 kDa and the isoelectric point was 9.28. The alignment showed that AcPI had a high identity with CsPIC2 (78.6%), AoPI (77.4%), OrcPI (75.7%) and HPI2 (72.4%). Quantitative real-time polymerase chain reaction (qRT-PCR) analyses in different tissues showed that the expression pattern of AcPI was different from the B-class genes in eudicots. AcPI was expressed in all the tissues investigated. The expression level was very low in fruit stems, bracts, leaves and sepals, high in petals and carpels, and moderate in apical meristems, flesh and stamens. The qRT-PCR analyses in different stages indicated that the expression of AcPI reached the highest level at 40 days after flower inducement, when the multiple fruit and floral organs were forming. It proved the important role of AcPI in floral organs and fruit development. The 35S::AcPI transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.
Keywords: pineapple; flower development; PI-like; cloning; expression; transformation
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Lv, L.-L.; Duan, J.; Xie, J.-H.; Liu, Y.-G.; Wei, C.-B.; Liu, S.-H.; Zhang, J.-X.; Sun, G.-M. Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr). Int. J. Mol. Sci. 2012, 13, 1039-1053.
Lv L-L, Duan J, Xie J-H, Liu Y-G, Wei C-B, Liu S-H, Zhang J-X, Sun G-M. Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr). International Journal of Molecular Sciences. 2012; 13(1):1039-1053.
Lv, Ling-Ling; Duan, Jun; Xie, Jiang-Hui; Liu, Yu-Ge; Wei, Chang-Bin; Liu, Sheng-Hui; Zhang, Jian-Xia; Sun, Guang-Ming. 2012. "Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr)." Int. J. Mol. Sci. 13, no. 1: 1039-1053.