Int. J. Mol. Sci. 2011, 12(7), 4435-4440; doi:10.3390/ijms12074435
Article

Isolation and Characterization of Microsatellite Loci in the Chinese Cobra Naja atra (Elapidae)

1email, 2email, 3email, 2email and 2,* email
Received: 7 June 2011; in revised form: 1 July 2011 / Accepted: 4 July 2011 / Published: 7 July 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We characterize thirteen polymorphic microsatellite loci isolated from Naja atra genomic libraries, which were enriched for AC-motif microsatellites. The thirteen loci were screened on a group of 48 individuals from two populations, one in Yong’an and the other in Ganzhou. These markers revealed a relatively high degree of genetic diversity (4–12 alleles per locus) and heterozygosity (Ho ranged from 0.213–0.854 and He ranged from 0.301–0.838). Tests for departure from Hardy-Weinberg equilibrium and for linkage disequilibrium were conducted for each of the two populations separately. After sequential Bonferroni correction, none of the 13 loci showed significant departures from Hardy-Weinberg equilibrium. Hierarchical analysis of molecular variance indicated that a small but significant (P < 0.001) proportion (16.0%) of the total variation in the microsatellite DNA data were attributable to differences among populations, indicating geographical structuring and restricted gene flow. It could be attributable to the Wuyi mountains in the area having a sufficiently isolating effect to significantly reduce gene flow. Our microsatellite data also showed a low Nm (1.31) value in the two populations from mainland China. Thus, the Yong’an and Ganzhou populations could be treated as distinct evolutionarily significant units (ESUs). The high level of polymorphism revealed by these microsatellite markers will be useful for the study of gene flow, population structure and evolutionary history of N. atra.
Keywords: Naja atra; Elapidae; microsatellites; PCR primers; polymorphism
PDF Full-text Download PDF Full-Text [250 KB, uploaded 19 June 2014 03:52 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Lin, L.-H.; Mao, L.-X.; Luo, X.; Qu, Y.-F.; Ji, X. Isolation and Characterization of Microsatellite Loci in the Chinese Cobra Naja atra (Elapidae). Int. J. Mol. Sci. 2011, 12, 4435-4440.

AMA Style

Lin L-H, Mao L-X, Luo X, Qu Y-F, Ji X. Isolation and Characterization of Microsatellite Loci in the Chinese Cobra Naja atra (Elapidae). International Journal of Molecular Sciences. 2011; 12(7):4435-4440.

Chicago/Turabian Style

Lin, Long-Hui; Mao, Lu-Xi; Luo, Xia; Qu, Yan-Fu; Ji, Xiang. 2011. "Isolation and Characterization of Microsatellite Loci in the Chinese Cobra Naja atra (Elapidae)." Int. J. Mol. Sci. 12, no. 7: 4435-4440.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert