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Int. J. Mol. Sci. 2011, 12(7), 4190-4205; doi:10.3390/ijms12074190
Article

Teucrium Plant Species as Natural Sources of Novel Anticancer Compounds: Antiproliferative, Proapoptotic and Antioxidant Properties

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Received: 11 April 2011; in revised form: 3 June 2011 / Accepted: 14 June 2011 / Published: 27 June 2011
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: This study deals with total phenolic content, antiproliferative and proapoptotic activity of methanolic extracts from different Teucrium species and the effect on the prooxidant/antioxidant status in HCT-116 cells. The total phenolic content of the extracts was measured spectrophotometricaly and the obtained results ranged from 56.62 mg/g to 172.50 mg GA/g. The antiproliferative activity of methanolic extracts from different Teucrium species was determined using MTT cell viability assay, where IC50 value was used as a parameter for cytotoxicity. The type of cell death was explored by fluorescence microscopy using the acridin orange/ethidium bromide method. MTT assay showed that all extracts significantly reduced cell viability in a dose-dependent manner, with very low IC50 values. The highest content of phenolic compounds and the best cytotoxic activity on HCT-116 cells after 24 h of exposure was in T. chamaedrys extract, with IC50 values of 5.48 × 10−9 µg/mL. After 72 h, methanolic extract of T. arduini appeared to have the best cytotoxic activity on HCT-116, with IC50 values of 0.37 µg/mL. Treatments caused typical apoptotic morphological changes in HCT-116 cells and showed a high percentage of apoptotic cells. The results of the presented research indicate that some Teucrium extracts are a very rich source of phenols, which may directly contribute to high antiproliferative and proapoptotic activity.
Keywords: acridin orange/ethidium bromide assay; antiprolifertative activity; antioxidants; MTT assay; phenols; Teucrium acridin orange/ethidium bromide assay; antiprolifertative activity; antioxidants; MTT assay; phenols; Teucrium
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Stankovic, M.S.; Curcic, M.G.; Zizic, J.B.; Topuzovic, M.D.; Solujic, S.R.; Markovic, S.D. Teucrium Plant Species as Natural Sources of Novel Anticancer Compounds: Antiproliferative, Proapoptotic and Antioxidant Properties. Int. J. Mol. Sci. 2011, 12, 4190-4205.

AMA Style

Stankovic MS, Curcic MG, Zizic JB, Topuzovic MD, Solujic SR, Markovic SD. Teucrium Plant Species as Natural Sources of Novel Anticancer Compounds: Antiproliferative, Proapoptotic and Antioxidant Properties. International Journal of Molecular Sciences. 2011; 12(7):4190-4205.

Chicago/Turabian Style

Stankovic, Milan S.; Curcic, Milena G.; Zizic, Jovana B.; Topuzovic, Marina D.; Solujic, Slavica R.; Markovic, Snezana D. 2011. "Teucrium Plant Species as Natural Sources of Novel Anticancer Compounds: Antiproliferative, Proapoptotic and Antioxidant Properties." Int. J. Mol. Sci. 12, no. 7: 4190-4205.


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