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Int. J. Mol. Sci. 2011, 12(6), 4104-4119; doi:10.3390/ijms12064104
Technical Note

Novel Polymorphic Microsatellite Loci for the Korean Black Scraper (Thamnaconus modestus), and Their Application to the Genetic Characterization of Wild and Farmed Populations

1,* , 2
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1 New Strategy Research Center, National Fisheries Research and Development Institute, Busan 619-705, Korea 2 Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-902, Korea 3 Genetics and Breeding Research Center, National Fisheries Research and Development Institute, Gyeongsangnamdo 656-842, Korea
* Author to whom correspondence should be addressed.
Received: 11 April 2011 / Revised: 10 June 2011 / Accepted: 13 June 2011 / Published: 20 June 2011
(This article belongs to the Special Issue Advances in Molecular Ecology)
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Abstract

In this study, we developed 20 polymorphic microsatellite markers for the Korean black scraper, Thamnaconus modestus (Günther, 1877), Monacanthidae, and used them to compare allelic variation between wild and hatchery populations in Korea. All loci were readily amplified and demonstrated allelic variability, with the number of alleles ranging from 5–35 in the wild population and 5–22 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, as 0.74 and 0.80 in the hatchery samples and 0.78 and 0.81 in the wild ones. These results indicate lower genetic variability in the hatchery population than in the wild population and minor, but significant, genetic differentiation between the two populations (FST = 0.005, P < 0.01). Additionally, cross-amplification was tested in another monacanthid species, Stephanolepis cirrhifer; many loci were found that yielded useful information. The high degree of polymorphism exhibited by the 20 microsatellites will be useful in future aquaculture and population genetic studies for developing conservation and management plans.
Keywords: Korean black scraper; Thamnaconus modestus; microsatellite loci; genetic marker; genetic variability Korean black scraper; Thamnaconus modestus; microsatellite loci; genetic marker; genetic variability
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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An, H.S.; Kim, E.M.; Lee, J.W.; Dong, C.M.; Lee, B.I.; Kim, Y.C. Novel Polymorphic Microsatellite Loci for the Korean Black Scraper (Thamnaconus modestus), and Their Application to the Genetic Characterization of Wild and Farmed Populations. Int. J. Mol. Sci. 2011, 12, 4104-4119.

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