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• Sun, D
• Sun, Y
• Wang, R
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• Wang, R
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• Sun, D
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Int. J. Mol. Sci. 2011, 12(6), 4021-4026; doi:10.3390/ijms12064021

Article

Development of 30 Novel Polymorphic Expressed Sequence Tags (EST)-Derived Microsatellite Markers for the Miiuy Croaker, Miichthys miiuy

Tianjun Xu, Dianqiao Sun, Yuena Sun and Rixin Wang*

Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China

* Author to whom correspondence should be addressed.

Received: 17 February 2011; in revised form: 6 May 2011 / Accepted: 20 May 2011 / Published: 15 June 2011

(This article belongs to the Section Molecular Diagnostics)

Download PDF Full-Text [149 KB, uploaded 15 June 2011 17:13 CEST]

Abstract: Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 30 polymorphic microsatellite markers from 5053 ESTs of the Miichthys miiuy. Out of 123 EST derived microsatellites for which PCR primers were designed, 30 loci were polymorphic in 30 individuals from a single natural population with 2–13 alleles per locus. The observed and expected heterozygosities were from 0.1024 to 0.7917 and from 0.2732 to 0.8845, respectively. Nine loci deviated from the Hardy-Weinberg equilibrium, and linkage disequilibrium was significant between 22 pairs of loci. These polymorphic microsatellite loci will be useful for genetic diversity analysis and molecule-assisted breeding for M. miiuy.

Keywords: microsatellite; Expressed sequence tags (ESTs); Miichthys miiuy

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