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Int. J. Mol. Sci. 2011, 12(5), 2842-2852; doi:10.3390/ijms12052842

Serine Proteases-Like Genes in the Asian Rice Gall Midge Show Differential Expression in Compatible and Incompatible Interactions with Rice

1
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
2
Acharya N. G. Ranga Agricultural University, Rajendranagar, Hyderabad 500030, India
3
Directorate of Rice Research, Rajendranagar, Hyderabad 500030, India
*
Author to whom correspondence should be addressed.
Received: 18 February 2011 / Revised: 21 March 2011 / Accepted: 12 April 2011 / Published: 29 April 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The Asian rice gall midge, Orseolia oryzae (Wood-Mason), is a serious pest of rice. Investigations into the gall midge-rice interaction will unveil the underlying molecular mechanisms which, in turn, can be used as a tool to assist in developing suitable integrated pest management strategies. The insect gut is known to be involved in various physiological and biological processes including digestion, detoxification and interaction with the host. We have cloned and identified two genes, OoprotI and OoprotII, homologous to serine proteases with the conserved His87, Asp136 and Ser241 residues. OoProtI shared 52.26% identity with mosquito-type trypsin from Hessian fly whereas OoProtII showed 52.49% identity to complement component activated C1s from the Hessian fly. Quantitative real time PCR analysis revealed that both the genes were significantly upregulated in larvae feeding on resistant cultivar than in those feeding on susceptible cultivar. These results provide an opportunity to understand the gut physiology of the insect under compatible or incompatible interactions with the host. Phylogenetic analysis grouped these genes in the clade containing proteases of phytophagous insects away from hematophagous insects.
Keywords: biotype; chymotrypsin; insect-plant interaction; phytophagous insects; real time PCR; trypsin biotype; chymotrypsin; insect-plant interaction; phytophagous insects; real time PCR; trypsin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sinha, D.K.; Lakshmi, M.; Anuradha, G.; Rahman, S.J.; Siddiq, E.A.; Bentur, J.S.; Nair, S. Serine Proteases-Like Genes in the Asian Rice Gall Midge Show Differential Expression in Compatible and Incompatible Interactions with Rice. Int. J. Mol. Sci. 2011, 12, 2842-2852.

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