Calpain Activator Dibucaine Induces Platelet Apoptosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies and Reagents
2.2. Platelet Preparation
2.3. Platelet Aggregation Assay
2.4. ΔΨm Measurement
2.5. PS Externalization Assay
2.6. Platelet Surface Staining
2.7. Western Blot Analysis
2.8. Confocal Microscopy
2.9. Caspase-3 Activity Assay
3. Results and Discussion
3.1. Dibucaine Dose-Dependently Induces Depolarization of ΔΨm in Platelets
3.2. Dibucaine Elicits Up-regulation of Bax and Bak, and Down-regulation of Bcl-2 and Bcl-XL in Platelets
3.3. Caspase-3 Is Activated in Platelets Treated with Dibucaine
3.4. Dibucaine Induces PS Exposure in Platelets
3.5. Dibucaine-Induced Platelet Apoptosis Is Independent of GPIbα Shedding
3.6. Dibucaine Does Not Induce Platelet Activation
3.7. Dibucaine Impairs Platelet Function
4. Conclusions
ijms-12-02125-s001.pdf
Acknowledgments
References
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Zhang, W.; Liu, J.; Sun, R.; Zhao, L.; Du, J.; Ruan, C.; Dai, K. Calpain Activator Dibucaine Induces Platelet Apoptosis. Int. J. Mol. Sci. 2011, 12, 2125-2137. https://doi.org/10.3390/ijms12042125
Zhang W, Liu J, Sun R, Zhao L, Du J, Ruan C, Dai K. Calpain Activator Dibucaine Induces Platelet Apoptosis. International Journal of Molecular Sciences. 2011; 12(4):2125-2137. https://doi.org/10.3390/ijms12042125
Chicago/Turabian StyleZhang, Weilin, Jun Liu, Ruichen Sun, Lili Zhao, Juan Du, Changgeng Ruan, and Kesheng Dai. 2011. "Calpain Activator Dibucaine Induces Platelet Apoptosis" International Journal of Molecular Sciences 12, no. 4: 2125-2137. https://doi.org/10.3390/ijms12042125