Next Article in Journal
Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro
Previous Article in Journal
Evaluation of Potential Reference Genes for Relative Quantification by RT-qPCR in Different Porcine Tissues Derived from Feeding Studies
Int. J. Mol. Sci. 2011, 12(3), 1735-1743; doi:10.3390/ijms12031735
Article

Synthesis of Molecularly Imprinted Polymers for Amino Acid Derivates by Using Different Functional Monomers

* ,
,
 and
*
Received: 14 January 2011 / Revised: 1 February 2011 / Accepted: 1 March 2011 / Published: 7 March 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [342 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

Fmoc-3-nitrotyrosine (Fmoc-3-NT) molecularly imprinted polymers (MIPs) were synthesized to understand the influence of several functional monomers on the efficiency of the molecular imprinting process. Acidic, neutral and basic functional monomers, such as acrylic acid (AA), methacrylic acid (MAA), methacrylamide (MAM), 2-vinylpyridine (2-VP), 4-vinylpyridine (4-VP), have been used to synthesize five different polymers. In this study, the MIPs were tested in batch experiments by UV-visible spectroscopy in order to evaluate their binding properties. The MIP prepared with 2-VP exhibited the highest binding affinity for Fmoc-3NT, for which Scatchard analysis the highest association constant (2.49 × 104 M−1) was obtained. Furthermore, titration experiments of Fmoc-3NT into acetonitrile solutions of 2-VP revealed a stronger bond to the template, such that a total interaction is observed. Non-imprinted polymers as control were prepared and showed no binding affinities for Fmoc-3NT. The results are indicative of the importance of ionic bonds formed between the –OH residues of the template molecule and the pyridinyl groups of the polymer matrix. In conclusion, 2-VP assists to create a cavity which allows better access to the analytes.
Keywords: molecularly imprinted polymer (MIP); Fmoc-3-nitrotyrosine (Fmoc-3NT); 2-vinylpyridine (2-VP); recognition mechanism molecularly imprinted polymer (MIP); Fmoc-3-nitrotyrosine (Fmoc-3NT); 2-vinylpyridine (2-VP); recognition mechanism
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Scorrano, S.; Mergola, L.; Del Sole, R.; Vasapollo, G. Synthesis of Molecularly Imprinted Polymers for Amino Acid Derivates by Using Different Functional Monomers. Int. J. Mol. Sci. 2011, 12, 1735-1743.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert