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Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro
Department of Sports Medicine and Arthroscopic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China
Department of Orthopedics, The Sixth Affiliated People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
* Author to whom correspondence should be addressed.
Received: 31 December 2010; in revised form: 22 February 2011 / Accepted: 1 March 2011 / Published: 7 March 2011
Abstract: The purpose of this study was to investigate the feasibility and advantages of constructing a novel tissue engineering bone, using β-tricalcium phosphate (β-TCP) and rat bone marrow mesenchymal stem cells (MSCs), modified with human bone morphogenetic protein 2 gene (hBMP2) and human vascular endothelial growth factor 165 gene (hVEGF165), through lentiviral transfection. Both genes were successfully co-expressed in the co-transfection group for up to eight weeks confirmed by enzyme-linked immunosorbent assay (ELISA). After seeding MSCs onto the scaffolds, scanning electron microscopy (SEM) observation showed that MSCs grew and proliferated well in co-transfection group at 7 and 14 days. There was no significant difference among all the groups in hoechst DNA assay for cell proliferation for 14 days after cell seeding (P > 0.05), but the highest alkaline phosphatase (ALP) activity was observed in the co-transfection group at 14 days after cell seeding (p < 0.01). These results demonstrated that it was advantageous to construct tissue engineering bone using β-TCP combined with MSCs lentivirally co-transfected with BMP2 and VEGF165, providing an innovative way for treating bone defects.
Keywords: bone morphogenetic protein 2; co-transfection; lentiviral vector; tissue engineering bone; vascular endothelial growth factor; β-tricalcium phosphate (β-TCP)
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Cite This Article
MDPI and ACS Style
Jiang, J.; Fan, C.-Y.; Zeng, B.-F. Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro. Int. J. Mol. Sci. 2011, 12, 1744-1755.
Jiang J, Fan C-Y, Zeng B-F. Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro. International Journal of Molecular Sciences. 2011; 12(3):1744-1755.
Jiang, Jia; Fan, Cun-Yi; Zeng, Bing-Fang. 2011. "Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro." Int. J. Mol. Sci. 12, no. 3: 1744-1755.