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Int. J. Mol. Sci. 2011, 12(3), 1744-1755; doi:10.3390/ijms12031744
Article

Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro

1
,
2
 and
2,*
1 Department of Sports Medicine and Arthroscopic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China 2 Department of Orthopedics, The Sixth Affiliated People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
* Author to whom correspondence should be addressed.
Received: 31 December 2010 / Revised: 22 February 2011 / Accepted: 1 March 2011 / Published: 7 March 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The purpose of this study was to investigate the feasibility and advantages of constructing a novel tissue engineering bone, using β-tricalcium phosphate (β-TCP) and rat bone marrow mesenchymal stem cells (MSCs), modified with human bone morphogenetic protein 2 gene (hBMP2) and human vascular endothelial growth factor 165 gene (hVEGF165), through lentiviral transfection. Both genes were successfully co-expressed in the co-transfection group for up to eight weeks confirmed by enzyme-linked immunosorbent assay (ELISA). After seeding MSCs onto the scaffolds, scanning electron microscopy (SEM) observation showed that MSCs grew and proliferated well in co-transfection group at 7 and 14 days. There was no significant difference among all the groups in hoechst DNA assay for cell proliferation for 14 days after cell seeding (P > 0.05), but the highest alkaline phosphatase (ALP) activity was observed in the co-transfection group at 14 days after cell seeding (p < 0.01). These results demonstrated that it was advantageous to construct tissue engineering bone using β-TCP combined with MSCs lentivirally co-transfected with BMP2 and VEGF165, providing an innovative way for treating bone defects.
Keywords: bone morphogenetic protein 2; co-transfection; lentiviral vector; tissue engineering bone; vascular endothelial growth factor; β-tricalcium phosphate (β-TCP) bone morphogenetic protein 2; co-transfection; lentiviral vector; tissue engineering bone; vascular endothelial growth factor; β-tricalcium phosphate (β-TCP)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Jiang, J.; Fan, C.-Y.; Zeng, B.-F. Experimental Construction of BMP2 and VEGF Gene Modified Tissue Engineering Bone in Vitro. Int. J. Mol. Sci. 2011, 12, 1744-1755.

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