Next Article in Journal
Combined 3D-QSAR Modeling and Molecular Docking Studies on Pyrrole-Indolin-2-ones as Aurora A Kinase Inhibitors
Previous Article in Journal
Light-Emitting Devices with Conjugated Polymers
Int. J. Mol. Sci. 2011, 12(3), 1595-1604; doi:10.3390/ijms12031595
Article

Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine

1,2,†,* , 1,†
, 1
, 1
, 1
, 1
 and 1,*
Received: 14 January 2011; in revised form: 1 February 2011 / Accepted: 17 February 2011 / Published: 1 March 2011
(This article belongs to the Section Molecular Pathology)
View Full-Text   |   Download PDF [268 KB, updated 19 June 2014; original version uploaded 19 June 2014]   |   Browse Figures
Abstract: Pancreatic cancer is one of the most lethal malignancies with poor prognosis. Previously, we found that a subpopulation of cancer stem cells (CSCs) in the Panc-1 pancreatic cancer cell line could propagate to form spheres. Here we characterized the malignant phenotypes of the pancreatic cancer stem CD44+/CD24+ cells, which were enriched under sphere forming conditions as analyzed by flow cytometry. These cells demonstrated increased resistance to gemcitabine and increased migration ability. Moreover, these cells exhibited epithelial to mesenchymal transition characterized by a decreased level of the epithelial marker E-cadherin and an increased level of the mesenchymal marker vimentin. Notably, abnormal expression of Bmi-1, ABCG2, Cyclin D1 and p16 were found in Panc-1 CSCs. Our results suggest that targeted inhibition of CSCs represents a novel therapeutic approach to overcome chemoresistance and metastasis of pancreatic cancer.
Keywords: pancreatic cancer; Bmi-1; chemoresistance; invasion; tumorigenesis pancreatic cancer; Bmi-1; chemoresistance; invasion; tumorigenesis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Yin, T.; Wei, H.; Gou, S.; Shi, P.; Yang, Z.; Zhao, G.; Wang, C. Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine. Int. J. Mol. Sci. 2011, 12, 1595-1604.

AMA Style

Yin T, Wei H, Gou S, Shi P, Yang Z, Zhao G, Wang C. Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine. International Journal of Molecular Sciences. 2011; 12(3):1595-1604.

Chicago/Turabian Style

Yin, Tao; Wei, Hongji; Gou, Shanmiao; Shi, Pengfei; Yang, Zhiyong; Zhao, Gang; Wang, Chunyou. 2011. "Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine." Int. J. Mol. Sci. 12, no. 3: 1595-1604.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert