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N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis
Environment and Biotechnology Department, Refining and Manufacturing Research Institute, CPC Corporation, Taiwan, Chia-yi, Taiwan
Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweishan, Taoyuan, Taiwan
Department of Chemical Engineering, Mingchi University of Technology, Taishan, New Taipei, Taiwan
Graduate School of Biochemical Engineering, Mingchi University of Technology, Taishan, New Taipei, Taiwan
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 3 December 2010; in revised form: 15 February 2011 / Accepted: 15 February 2011 / Published: 17 February 2011
(This article belongs to the Special Issue Chitins
Abstract: A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation.
Keywords: CDFs; Chitinibacter tainanensis; chitin; NAG; fermentation
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Chen, J.-K.; Shen, C.-R.; Yeh, C.-H.; Fang, B.-S.; Huang, T.-L.; Liu, C.-L. N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis. Int. J. Mol. Sci. 2011, 12, 1187-1195.
Chen J-K, Shen C-R, Yeh C-H, Fang B-S, Huang T-L, Liu C-L. N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis. International Journal of Molecular Sciences. 2011; 12(2):1187-1195.
Chen, Jeen-Kuan; Shen, Chia-Rui; Yeh, Chao-Hsien; Fang, Bing-Shiun; Huang, Tung-Li; Liu, Chao-Lin. 2011. "N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis." Int. J. Mol. Sci. 12, no. 2: 1187-1195.