Int. J. Mol. Sci. 2011, 12(2), 1030-1040; doi:10.3390/ijms12021030
Article

Chemically Induced Breast Tumors in Rats Are Detectable in Early Stages by Contrast Enhanced Magnetic Resonance Imaging but Not by Changes in the Acute-Phase Reactants in Serum

Shahram Golbabapour 1 email, Wei Wei Pang 2 email, John George 3 email, Thanikachalam Pasupati 4 email, Puteri Shafinaz Abdul-Rahman 1,2 email and Onn Haji Hashim 1,2,* email
1 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia 2 University of Malaya Centre for Proteomics Research, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia 3 Department of Biomedical Imaging, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia 4 Gribbles Pathology, Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 20 December 2010; in revised form: 4 February 2011 / Accepted: 4 February 2011 / Published: 7 February 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: The present study was undertaken to develop a rat model for monitoring the early development of breast cancer. Twelve female rats were divided into two groups of six rats that were either treated with N-methyl-N-nitrosourea to induce breast cancer or with bacterial lipopolysaccharide to induce inflammation. Serum samples taken from the rats prior to the treatment were used as controls. By the 14th week, presence of the tumor was detectable by contrast enhanced magnetic resonance imaging and confirmed by histopathology. When the serum proteins of the rats were examined by 2-dimensional electrophoresis (2-DE), no difference could be detected in the profiles of all proteins before and 18 weeks after administration of N-methyl-N-nitrosourea. However, higher expression of alpha-1B glycoprotein was detectable by 2-DE in serum samples of rats at the 18th week post-treatment with lipopolysaccharide.
Keywords: acute-phase response; breast cancer; proteomics; magnetic resonance imaging

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MDPI and ACS Style

Golbabapour, S.; Pang, W.W.; George, J.; Pasupati, T.; Abdul-Rahman, P.S.; Hashim, O.H. Chemically Induced Breast Tumors in Rats Are Detectable in Early Stages by Contrast Enhanced Magnetic Resonance Imaging but Not by Changes in the Acute-Phase Reactants in Serum. Int. J. Mol. Sci. 2011, 12, 1030-1040.

AMA Style

Golbabapour S, Pang WW, George J, Pasupati T, Abdul-Rahman PS, Hashim OH. Chemically Induced Breast Tumors in Rats Are Detectable in Early Stages by Contrast Enhanced Magnetic Resonance Imaging but Not by Changes in the Acute-Phase Reactants in Serum. International Journal of Molecular Sciences. 2011; 12(2):1030-1040.

Chicago/Turabian Style

Golbabapour, Shahram; Pang, Wei Wei; George, John; Pasupati, Thanikachalam; Abdul-Rahman, Puteri Shafinaz; Hashim, Onn Haji. 2011. "Chemically Induced Breast Tumors in Rats Are Detectable in Early Stages by Contrast Enhanced Magnetic Resonance Imaging but Not by Changes in the Acute-Phase Reactants in Serum." Int. J. Mol. Sci. 12, no. 2: 1030-1040.

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